Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD+-dependent polynucleotide ligases. [PDF]
Proc Natl Acad Sci U S A, 2017 Significance This season marks the 50th anniversary of the discovery of polynucleotide ligases, the sine qua non enzymes of nucleic acid repair and the enabling reagents of molecular biology, reported in a ...
Unciuleac MC, Goldgur Y, Shuman S.
europepmc +2 more sources
Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis [PDF]
, 1976 DNA molecules cut with endonuclease R Eco RI can be joined at Eco RI cleavage sites by incubation with polynucleotide ligase. In order to define the optimum conditions for this reaction, linear Simian Virus 40 DNA molecules (SV40(LRI)) produced by ...
De Vries, F. A. J. +2 more
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A novel high- Throughput activity assay for the Trypanosoma brucei editosome enzyme REL1 and other RNA ligases [PDF]
, 2015 The protist parasite Trypanosoma brucei causes Human African trypanosomiasis (HAT), which threatens millions of people in sub-Saharan Africa. Without treatment the infection is almost always lethal.
Schnaufer, Achim; id_orcid +11 more
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Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]
, 2006 The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Bullard, Desmond R., Bowater, Richard P.
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Closing the gap on DNA ligase [PDF]
, 1996 The crystal structure of T7 DNA ligase complexed with ATP illuminates the mechanism of covalent catalysis by a superfamily of nucleotidyl transferases that includes the ATP-dependent polynucleotide ligases and the GTP-dependent mRNA capping ...
Shuman, Stewart
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Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans [PDF]
, 2007 BACKGROUND: Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans genome after extremely high doses ...
Hubscher, U +11 more
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Identification and Characterization of Small Molecule Inhibitors of Polynucleotide Kinase 3'-Phosphatase [PDF]
, 2012 DNA lesions arise constantly in cells and are repaired by a variety of DNA repair pathways. Polynucleotide kinase 3’-phosphatase (PNKP) aids repair by phosphorylating 5’-hydroxyl DNA termini and dephosphorylating 3’-phosphate DNA termini for the ...
Moatti, Nathalie
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Polynucleotide kinase as a potential target for enhancing cytotoxicity by ionizing radiation and topoisomerase I inhibitors. [PDF]
, 2008 The cytotoxicity of many antineoplastic agents is due to their capacity to damage DNA and there is evidence indicating that DNA repair contributes to the cellular resistance to such agents.
Glover, JN +6 more
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Biodiscovery of Novel RNA Ligases with Biotechnological Potential - New Insights into the RNA Ligase 3 Family [PDF]
, 2020 Polynucleotide ligases constitute a class of enzymes which catalyse the formation of phosphodiester bonds between proximal 5’-PO4 and 3’-OH groups in DNA, RNA and DNA/RNA-hybrid substrates.
Gábor, Hannah Ildikó Johnstad
core
Branchiostoma Floridae Has Separate Healing and Sealing Enzymes for 5′-phosphate RNA Ligation [PDF]
, 2010 Animal cells have two tRNA splicing pathways: (i) a 5′-P ligation mechanism, where the 5′-phosphate of the 3′ tRNA half becomes the junction phosphate of the new phosphodiester linkage, and (ii) a 3′-P ligation process, in which the 3′-phosphate of the 5′
Beier, Hildburg +9 more
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