Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD+-dependent polynucleotide ligases. [PDF]
Proc Natl Acad Sci U S A, 2017 Significance This season marks the 50th anniversary of the discovery of polynucleotide ligases, the sine qua non enzymes of nucleic acid repair and the enabling reagents of molecular biology, reported in a ...
Unciuleac MC, Goldgur Y, Shuman S.
europepmc +2 more sources
Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis [PDF]
, 1976 DNA molecules cut with endonuclease R Eco RI can be joined at Eco RI cleavage sites by incubation with polynucleotide ligase. In order to define the optimum conditions for this reaction, linear Simian Virus 40 DNA molecules (SV40(LRI)) produced by ...
De Vries, F. A. J. +2 more
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Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases. [PDF]
, 2013 Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 ...
Canning, P +40 more
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Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]
, 2006 The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Bullard, Desmond R., Bowater, Richard P.
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Temperature adaptation of DNA ligases from psychrophilic organisms [PDF]
, 2019 DNA ligases operating at low temperatures have potential advantages for use in biotechnological applications. For this reason, we have characterized the temperature optima and thermal stabilities of three minimal Lig E-type ATP-dependent DNA ligase ...
Williamson, Adele Kim +2 more
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Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans [PDF]
, 2007 BACKGROUND: Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans genome after extremely high doses ...
Hubscher, U +15 more
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NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor [PDF]
, 2003 Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli.
Bullard, Desmond +6 more
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Polynucleotide length differences in T. pallidum Nichols clones.
, 2023 Polynucleotide length differences in T. pallidum Nichols clones.
Diane G. Edmondson (9515284) +4 more
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Synthesis of a novel polynucleotide: potential R-L model [PDF]
, 1988 Synthesis of a novel polynucleotide alternating B and Z segments is described. This is the first report of a polynucleotide where the double helix undergoes change in handedness after every half a ...
Mishra, R. K., Brahmachari, S. K.
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Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures [PDF]
European Journal of Biochemistry, 1971 A polynucleotide ligase has been detected and purified over 300‐fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat‐resistant protein factor present in the crude extract.
S, Spadari, G, Ciarrocchi, A, Falaschi
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