Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay
Frontiers in Genetics, 2014 Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons +1 more
doaj +2 more sources
Two distinct polynucleotide ligases from rat liver
FEBS Letters, 1975 Hirobumi Teraoka, Kinji Tsukada
exaly +2 more sources
Joining of simian virus 40 DNA molecules at endonuclease R Eco RI sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis [PDF]
, 1976 DNA molecules cut with endonuclease R Eco RI can be joined at Eco RI cleavage sites by incubation with polynucleotide ligase. In order to define the optimum conditions for this reaction, linear Simian Virus 40 DNA molecules (SV40(LRI)) produced by ...
De Vries, F. A. J. +2 more
core +1 more source
A novel high- Throughput activity assay for the Trypanosoma brucei editosome enzyme REL1 and other RNA ligases [PDF]
, 2015 The protist parasite Trypanosoma brucei causes Human African trypanosomiasis (HAT), which threatens millions of people in sub-Saharan Africa. Without treatment the infection is almost always lethal.
Schnaufer, Achim; id_orcid +11 more
core +2 more sources
Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans [PDF]
, 2007 BACKGROUND: Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans genome after extremely high doses ...
Hubscher, U +11 more
core +1 more source
, 2017 Significance This season marks the 50th anniversary of the discovery of polynucleotide ligases, the sine qua non enzymes of nucleic acid repair and the enabling reagents of molecular biology, reported in a ...
Stewart Shuman +2 more
core +1 more source
Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4
, 2006 The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Bullard, Desmond R., Bowater, Richard P.
core +1 more source
Biodiscovery of Novel RNA Ligases with Biotechnological Potential - New Insights into the RNA Ligase 3 Family [PDF]
, 2020 Polynucleotide ligases constitute a class of enzymes which catalyse the formation of phosphodiester bonds between proximal 5’-PO4 and 3’-OH groups in DNA, RNA and DNA/RNA-hybrid substrates.
Gábor, Hannah Ildikó Johnstad
core
Molecular Microbiology, EarlyView.Here, we demonstrate that polysaccharide chain length regulation plays a distinct role in enabling formation of a cyclic molecule important for the bacterial cell surface. However, this function is necessary, but not sufficient for the cyclic molecule's biosynthesis.
Joseph F. Carr +4 more
wiley +1 more source
Technologies for engineering repetitive DNA
Quantitative Biology, Volume 14, Issue 3, September 2026.Abstract Repetitive DNA, a fundamental architectural element of genomes, is widespread across organisms and comprises about 54% of the human genome. With advances in long‐read sequencing and bioinformatics approaches, highly repetitive sequences can now be characterized in depth.
Shuting Ma, Yali Cui, Yi Wu
wiley +1 more source