Results 21 to 30 of about 65 (61)
Improving iSpyMacCas9 multiplex genome editing in rice by CRISPR‐combo‐mediated BBM1 activation
The Plant Journal, Volume 126, Issue 5, June 2026.Significance Statement This study establishes iSpyMacCas9‐Combo as an expanded CRISPR‐Combo platform that enables efficient simultaneous genome editing and transcriptional activation in rice, particularly at AT‐rich or low‐efficiency target sites.
Innocent Byiringiro +5 more
wiley +1 more source
Advanced Science, Volume 13, Issue 29, 22 May 2026.This study reveals the molecular mechanism by which the transcription factor FgSge1 regulates mycotoxin biosynthesis and virulence in Fusarium graminearum. FgSge1 binds to the TAARGTTT cis‐element, enabling self‐activation. It recruits the SAGA complex, promotes histone acetylation, and facilitates jet‐like chromatin remodeling, thereby activating ...
Yueqi Zhang +6 more
wiley +1 more source
Advanced Science, Volume 13, Issue 26, 8 May 2026.In this study, we identified an NSUN6‐dependent m5C‐modified tsRNA, m5C‐tRF3b CysGCA‐23 (mtRC), that is downregulated in BC and inversely correlated with disease progression. Mechanistically, mtRC suppresses BC malignancy by stabilizing RBM4, attenuating glycolysis, and thereby limiting H3K18 lactylation–mediated activation of IL1RAP and VASH2 ...
Xiaoling Ying +16 more
wiley +1 more source
Generation of Cloned Sheep Lacking Galactose‐α1,3‐Galactose and N‐Glycolylneuraminic Acid Antigens
Xenotransplantation, Volume 33, Issue 3, May/June 2026.ABSTRACT Livestock have long been regarded as a potential source of donor organs to alleviate the global organ shortage for transplantation. Sheep have a similar anatomy to humans, providing the standard model for demonstrating biocompatibility and performance of biological heart valves to obtain regulatory approval for their use in transplantation ...
Sarah J. Appleby +9 more
wiley +1 more source
Preserving DNAzyme Stability and Activity in Stool Samples for Clinical Diagnostics
Chemistry–Methods, Volume 6, Issue 4, April 2026.A RNA‐cleaving DNAzymes often lose activity in stool due to extensive nuclease‐mediated degradation. This work introduces a simple heparin‐based treatment that preserves DNAzyme integrity and catalytic function in this highly challenging matrix, enabling reliable pathogen‐responsive signaling in stool samples.
Soyeon Lee +3 more
wiley +1 more source
Branchiostoma Floridae Has Separate Healing and Sealing Enzymes for 5′-phosphate RNA Ligation
, 2010 Animal cells have two tRNA splicing pathways: (i) a 5′-P ligation mechanism, where the 5′-phosphate of the 3′ tRNA half becomes the junction phosphate of the new phosphodiester linkage, and (ii) a 3′-P ligation process, in which the 3′-phosphate of the 5′
Beier, Hildburg +9 more
core +1 more source
Resurrecting the Activity of the RNA Subunit of Human RNase P
ChemBioChem, Volume 27, Issue 6, 27 March 2026.The catalytic RNA (H1 RNA) of the human nuclear tRNA processing enzyme RNase P has >105‐fold lower activity in the absence of its 11 protein cofactors than bacterial RNase P RNAs. By introducing mutations toward the bacterial consensus into its catalytic domain, a two and fivefold activity enhancement could be achieved by changes in the P4‐19/4 region ...
Dan Li +2 more
wiley +1 more source
Biochemical Characterization Of The Rtcb Family Of Rna Ligases In Bacteria [PDF]
, 2016 All known life relies on the proper transmission of genetic information through DNA and RNA to protein. The protection of this information is critical, and rejoining of broken DNA or RNA strands by ligases is essential for maintenance of genetic ...
Maughan, William
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Motorized DNAzymes Drive Enhanced Electrochemical Biosensing for Rapid Bacterial Detection
Small Methods, Volume 10, Issue 6, 24 March 2026.The micromotor DNAzyme analyzer (Motolyzer) enables sensitive electrochemical detection of Legionella pneumophila proteins in unprocessed samples with high specificity. Upon target recognition, DNAzymes actively release redox barcodes, which are detected on DNA‐functionalized electrodes.
Amir Ali Akhlaghi +9 more
wiley +1 more source
On the mechanism of the polynucleotide joining reaction [PDF]
, 1968 journal articlePolynucleotide-joining enzymes (ligases) have been identified in uninfected and phage-infected Escherichia coli (Gellert, 1967; Olivera and Lehman, 1967a; Zimmerman et al., 1967; Gefter et al., 1967; Weiss and Richardson, 1967a; Cozzarelli
Olivera, Baldomero M. +1 more
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