Results 21 to 30 of about 19,008 (171)

Closing the gap on DNA ligase

, 1996
The crystal structure of T7 DNA ligase complexed with ATP illuminates the mechanism of covalent catalysis by a superfamily of nucleotidyl transferases that includes the ATP-dependent polynucleotide ligases and the GTP-dependent mRNA capping ...
Shuman, Stewart
core   +1 more source

Identification and Characterization of Small Molecule Inhibitors of Polynucleotide Kinase 3'-Phosphatase [PDF]

, 2012
DNA lesions arise constantly in cells and are repaired by a variety of DNA repair pathways. Polynucleotide kinase 3’-phosphatase (PNKP) aids repair by phosphorylating 5’-hydroxyl DNA termini and dephosphorylating 3’-phosphate DNA termini for the ...
Moatti, Nathalie
core   +1 more source

Engineered Transformer Base Editor with Enhanced Editing Efficiency

Advanced Science, EarlyView.
A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen, Letong Liang, Rui Xu, Tianyi Wang, Hongyan Chen, Daru Lu   +5 more
wiley   +1 more source

Polynucleotide Evolution and Branching Processes [PDF]

, 1984
The theory of multitype branching processes is applied to the kinetics of polynucleotide replication. The results obtained are compared with the solutions of the deterministic differential equations from conventional chemical ...
Demetrius, L., Sigmund, K., Schuster, P., Karl Sigmund, Lloyd Demetrius, Peter Schuster   +5 more
core   +2 more sources

The Novel Viral Non‐Coding RNA LMP2A‐IT1 Promotes the Lytic Replication of EBV

Advanced Science, EarlyView.
Decades after its discovery, EBV's genome still harbours unannotated “dark matter”. LMP2A‐IT1, a novel EBV‐encoded transcript identified in this work, is strongly upregulated during lytic replication and promotes BZLF1 expression, likely through activation of inflammatory responses, thereby enhancing viral propagation in a self‐reinforcing positive ...
Zheng‐Zhou Lu, Jun‐Fei Wu, Nannan Zhu, Ming‐Hui Yang, Le‐Le Zhang, Yuan‐Tao Liu, Wei‐Jun Chen, Jin‐Kun Tang, Jia‐Wei Ge, Chao‐Bo Lei, Tao Zhang, Yu Wang, Chao‐Yun Huang, Qian Zhong, Mu‐Sheng Zeng, Yan Li   +15 more
wiley   +1 more source

Exploiting Phase Partitioning of a Giant Trimeric Fibrous Protein Scaffold to Potentiate Chitinase‐Driven Antifungal Activity

Small, EarlyView.
Cstalk, a giant fibrous protein, selectively partitions into the dextran‐rich phase in polyethylene glycol/dextran aqueous two‐phase systems, functioning as a recruiter for proteins of interest. This phase‐selective recruitment can be extended to fungal cell walls, enabling the targeted localization of enzymes and enhancing antifungal activity through ...
Ayasa Nagatani, Shogo Yoshimoto, Toki Taira, Rie Wakabayashi, Masahiro Goto, Katsutoshi Hori, Noriho Kamiya   +6 more
wiley   +1 more source

Human APOBEC3G suppresses homologous recombination and LIG4‐independent end joining in DNA double‐strand break repair

The FEBS Journal, EarlyView.
Chromosomal DNA double‐strand breaks (DSBs) are repaired by homologous recombination and nonhomologous end joining (NHEJ). Recent work has additionally established theta‐mediated end‐joining (TMEJ) as a mechanism for DSB joining. Cells lacking NHEJ and TMEJ can repair DSBs in a homology‐dependent manner.
Shinta Saito, Miori Murayama, Usaki Arai, Sekifumi Akatsu, Ami Yamamoto, Misaki Kadoguchi, Noritaka Adachi   +6 more
wiley   +1 more source

Dual‐Organelle Secretome Conjugation and Organ‐Uptake Tracking (DuO‐SCOUT) Enables Deep and Sensitive Mapping of the Secreted Proteins

Advanced Science, Volume 13, Issue 61, 3 November 2026.
A novel dual‐organelle proximity labeling platform, DuO‐SCOUT, decodes the complex landscape of systemic organ‐organ communication by capturing both classical and unconventional secretomes. Application in metabolic models maps the adipose‐to‐brain secretory relay, identifying selective extra‐hypothalamic sites for adipose‐derived factors and expanding ...
Fenglian Yang, Rui Qian, Jiaxing Song, Xingqi Meng, Jin Young Kim, Kwok On Lai, Li Wang, Liang Zhang   +7 more
wiley   +1 more source

POLYNUCLEOTIDE RESPONSIBLE OF HAPLOID INDUCTION IN MAIZE PLANTS AND RELATED PROCESSES

, 2015
The present invention concerns an isolated polynucleotide responsible of haploid induction in maize plants and related processes. Additionally, the invention relates to plants that have been genetically transformed with the polynucleotide of the ...
Widiez, Thomas, Rogowsky, Peter, Laffaire, Jean-Baptiste, Martinant, Jean-Pierre, Comadran, Jordi   +4 more
core   +4 more sources

Engineering Mosaic T7 Bacteriophages for Dual Peptide Display to Enable Cellular Targeting and Ligand‐Directed Protein Capture

Biotechnology Journal, Volume 21, Issue 11, November 2026.
Graphical Abstract and Lay Summary A mosaic T7 phage platform enables simultaneous display of two independent peptides on the same phage particle. The modular design integrates receptor targeting, protein capture, and tissue‐directed in vivo phage display, establishing a versatile methodology for multifunctional phage engineering, receptor ...
Luca Marchetti, Kristina Põšnograjeva, Prakash Lingasamy, Karlis Pleiko, Ivar Ilves, Tambet Teesalu   +5 more
wiley   +1 more source