Results 31 to 40 of about 1,923 (109)
Improving iSpyMacCas9 multiplex genome editing in rice by CRISPR‐combo‐mediated BBM1 activation
The Plant Journal, Volume 126, Issue 5, June 2026.Significance Statement This study establishes iSpyMacCas9‐Combo as an expanded CRISPR‐Combo platform that enables efficient simultaneous genome editing and transcriptional activation in rice, particularly at AT‐rich or low‐efficiency target sites.
Innocent Byiringiro +5 more
wiley +1 more source
On the mechanism of the polynucleotide joining reaction [PDF]
, 1968 journal articlePolynucleotide-joining enzymes (ligases) have been identified in uninfected and phage-infected Escherichia coli (Gellert, 1967; Olivera and Lehman, 1967a; Zimmerman et al., 1967; Gefter et al., 1967; Weiss and Richardson, 1967a; Cozzarelli
Olivera, Baldomero M. +1 more
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Advanced Science, Volume 13, Issue 29, 22 May 2026.This study reveals the molecular mechanism by which the transcription factor FgSge1 regulates mycotoxin biosynthesis and virulence in Fusarium graminearum. FgSge1 binds to the TAARGTTT cis‐element, enabling self‐activation. It recruits the SAGA complex, promotes histone acetylation, and facilitates jet‐like chromatin remodeling, thereby activating ...
Yueqi Zhang +6 more
wiley +1 more source
Synthetic Biology Methods to Optimise T4 RNA Ligase Activities [PDF]
, 2018 The T4 nucleic acid (NA) ligases repair breaks in nucleic acids, which can be double-stranded DNA, RNA or a combination of both. The bacteriophage T4 has 3 NA ligases, one is a DNA ligase (T4Dnl) and two are RNA ligases: T4 RNA ligase 1 (T4Rnl1) and T4 ...
Easey, Amy
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Advanced Science, Volume 13, Issue 26, 8 May 2026.In this study, we identified an NSUN6‐dependent m5C‐modified tsRNA, m5C‐tRF3b CysGCA‐23 (mtRC), that is downregulated in BC and inversely correlated with disease progression. Mechanistically, mtRC suppresses BC malignancy by stabilizing RBM4, attenuating glycolysis, and thereby limiting H3K18 lactylation–mediated activation of IL1RAP and VASH2 ...
Xiaoling Ying +16 more
wiley +1 more source
Characterization of an ATP-Dependent DNA Ligase Encoded by \u3ci\u3eChlorella\u3c/i\u3e Virus PBCV-1 [PDF]
, 1997 We report that Chlorella virus PBCV-1 encodes a 298-amino-acid ATP-dependent DNA ligase. The PBCV-1 enzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the ATP-dependent polynucleotide ligases and the GTP ...
Ho, C. Kiong +2 more
core +1 more source
Generation of Cloned Sheep Lacking Galactose‐α1,3‐Galactose and N‐Glycolylneuraminic Acid Antigens
Xenotransplantation, Volume 33, Issue 3, May/June 2026.ABSTRACT Livestock have long been regarded as a potential source of donor organs to alleviate the global organ shortage for transplantation. Sheep have a similar anatomy to humans, providing the standard model for demonstrating biocompatibility and performance of biological heart valves to obtain regulatory approval for their use in transplantation ...
Sarah J. Appleby +9 more
wiley +1 more source
Construction and screening of a pea root cDNA library [PDF]
, 1988 A cDNA library was synthesized using poly(A)(^+) RNA purified from the total RNA from roots of pea (Pisum sativum L.). Ten representative clones encoding abundant root proteins were isolated from the library after screening using colony hybridization ...
Choy, Andy Kwan Yan
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Understanding the Sequence Dependence of NHEJ Mediated Double-strand Break Repair, and Identification of Novel DNA Ligase Inhibitors and their Potential Use as Cancer Therapeutics [PDF]
, 2017 Repair of DNA breaks is essential for maintenance of genomic integrity. DNA double-strand breaks (DSBs) are considered as the most harmful DNA lesions within the cells which if left unrepaired or are misrepaired, can lead to a wide variety of genetic ...
Pandey, Monica
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La jonction d'extrémités non-homologues : une nouvelle fonction pour le complexe de ligature [PDF]
, 2012 Chez les vertébrés, la voie majoritaire de réparation des cassures double brin de l'ADN est la jonction d'extrémité non homologue (NHEJ). Afin d'initier la réparation, la protéine hétérodimérique Ku se lie aux extrémités de l'ADN et recrute la sous unité
Cottarel, Jessica
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