Results 31 to 40 of about 65 (61)
Evolved DNAzymes and Stable Activation Chemistry Enable High‐Efficiency DNA Ligation
In vitro selection of pre‐structured DNA libraries and chemical screening of alternative activation groups yielded trans‐acting DNAzymes with faster ligation rates and enhanced DNA substrate stability. The combination of C3S1 DNAzymes and phosphobenzimidazole‐activated DNA substrates provides improved DNA ligation performance, offering promise for ...
Connor Nurmi +3 more
wiley +1 more source
Synthetic Biology Methods to Optimise T4 RNA Ligase Activities [PDF]
The T4 nucleic acid (NA) ligases repair breaks in nucleic acids, which can be double-stranded DNA, RNA or a combination of both. The bacteriophage T4 has 3 NA ligases, one is a DNA ligase (T4Dnl) and two are RNA ligases: T4 RNA ligase 1 (T4Rnl1) and T4 ...
Easey, Amy
core
A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase
[EN] Bacteria and archaea contain a 2'-5' RNA ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl RNA termini, generating a 2',5'-phosphodiester bond. In our search for an RNA ligase able to circularize the monomeric linear replication
Daròs, José-Antonio +9 more
core +1 more source
Nanozymes Integrated Biochips Toward Smart Detection System
This review systematically outlines the integration of nanozymes, biochips, and artificial intelligence (AI) for intelligent biosensing. It details how their convergence enhances signal amplification, enables portable detection, and improves data interpretation.
Dongyu Chen +10 more
wiley +1 more source
GeTe exhibits intrinsically high carrier concentrations that limit its thermoelectric performance. Herein, CuAgSe alloying synergistically tunes the band structure, induces band flattening and valence‐band convergence, promotes Cu‐ion delocalization, and generates multiscale hierarchical structures to suppress phonon transport.
Junhang Park +13 more
wiley +1 more source
Construction and screening of a pea root cDNA library [PDF]
A cDNA library was synthesized using poly(A)(^+) RNA purified from the total RNA from roots of pea (Pisum sativum L.). Ten representative clones encoding abundant root proteins were isolated from the library after screening using colony hybridization ...
Choy, Andy Kwan Yan
core
Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair
Mutations in mitochondrial DNA (mtDNA) are implicated in a broad range of human diseases and in aging. Compared to nuclear DNA, mtDNA is more highly exposed to oxidative damage due to its proximity to the respiratory chain and the lack of protection ...
Nasser Tahbaz +2 more
core
Studies on Prokaryotic and Eukaryotic Polynucleotide Ligases
Made available in DSpace on 2014-12-14T07:14:45Z (GMT). No. of bitstreams: 1 7714926.pdf: 4224938 bytes, checksum: 8727bde8d854483297215c784ed8faf6 (MD5) Previous issue date: 1977Embargo set by: Seth Robbins for item 68256 Lift date: Forever Reason ...
Bedows, Elliott David
core
The multiple roles of A-type lamins in cellular aging, cell cycle progression and the DNA damage response. [PDF]
A-type lamins are a group of type V intermediate filaments whose main members are lamin A and C. Lamins A/C are components of the nuclear lamina and are encoded by the LMNA gene.
GIBBS-SEYMOUR, IAN,DAVID
core
Characterization of an ATP-Dependent DNA Ligase Encoded by \u3ci\u3eChlorella\u3c/i\u3e Virus PBCV-1
We report that Chlorella virus PBCV-1 encodes a 298-amino-acid ATP-dependent DNA ligase. The PBCV-1 enzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the ATP-dependent polynucleotide ligases and the GTP ...
Ho, C. Kiong +2 more
core

