Results 31 to 40 of about 3,773 (182)

High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing [PDF]

, 2013
Small RNA cloning and sequencing is uniquely positioned as a genome-wide approach to quantify miRNAs with single-nucleotide resolution. However, significant biases introduced by RNA ligation in current protocols lead to inaccurate miRNA quantification by
Emily M Anderson, Jerome E Lee, Kent Riemondy, Rui Yi, Zhaojie Zhang   +4 more
core   +1 more source

Global Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures at the Single‐Nick Level

Small, EarlyView.
Ligations of staple strands by ligase at 64 nick sites on DNA origami can be quantitatively mapped. While ligations preferentially occur at the trapezoid edges rather than at inner sites, excellent agreement between the experimental ligation yields and docking simulations suggests that this is a result of variations in the ligase docking probability ...
Konrad Hacker, Emilia Juricke, Carolin Münch, Antonio Suma, Adrian Keller, Yixin Zhang   +5 more
wiley   +1 more source

Method of identifying inhibitors of glutathione S-transferase (GST) gene expression [PDF]

, 1999
Complementary DNA and genomic clones for three variants of GST-.pi. are disclosed. It is demonstrated that certain of these variants are overexpressed in gliomas, thereby indicating an involvement with that form of cancer.
Akande, Olanike, Ali-Osman, Francis, Antoun, Gamil, Buolamwini, John K., Keller, Charles W., Lo, Hui-Wen, Lopez-Berestein, Gabriel   +6 more
core   +1 more source

Redox environment modulates aggregation of ataxin‐3 in vitro — Implications for drug screening of cysteine‐rich proteins

The FEBS Journal, EarlyView.
Redox environment modulates in vitro aggregation of Ataxin‐3, the protein implicated in spinocerebellar ataxia type 3. Reducing conditions stabilize native monomers and prevent aggregation, whereas oxidative conditions promote the formation of non‐native conformers and disulfide‐linked oligomers within the Josephin domain (JD).
Martyna Podlasiak, Martina Sollazzo, Elisa Monaca, Raffaele Sabbatella, Maria Agnese Morando, Oleg Chertkov, Laura Puglisi, Martina Mascellino, Anna Fricano, Sandra Macedo‐Ribeiro, Caterina Alfano   +10 more
wiley   +1 more source

Some observations on the cofactor requirement for partially purified DNA ligase from Escherichia coli [PDF]

, 1972
Farrando Sicilia, Jordi; Fuente Fuente ...
Richardson, Hershey, Olivera, Gefter, Gellert, Zimmerman, Little, Weiss, Meselson, Modrich, Gellert   +10 more
core   +1 more source

Technologies for engineering repetitive DNA

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract Repetitive DNA, a fundamental architectural element of genomes, is widespread across organisms and comprises about 54% of the human genome. With advances in long‐read sequencing and bioinformatics approaches, highly repetitive sequences can now be characterized in depth.
Shuting Ma, Yali Cui, Yi Wu
wiley   +1 more source

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts [PDF]

, 2016
Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states.
A Basters, A Kriz, AB Rode, AJ Jakobi, B Geering, B Weiss, BM Olivera, C Bieniossek, C Bieniossek, C Romier, C Schaffitzel, CP Chou, CV Robinson, D Busso, D Sari, D Schelshorn, DG Gibson, DJ Pitera, DN Gopaul, F Guo, F Leinert, G Bartani, G Gosset, G Kellenberger, H Alper, H Ye, HO Smith, J Komar, J Quan, J Torella, JA Chemler, JH Park, K Ballmer-Hofer, KB Mullis, LF Estrozi, LR Jarboe, LS Vijayachandran, M Folcher, M Gellert, M Haffke, M Haffke, M Meselson, MCY Chang, ML Diebold, ML Gefter, MZ Li, N Zheng, NR Cozzarelli, O Loeffelholz Von, PE Nisson, PJA Cock, R Patnaik, R Vincentelli, RJ Schulze, S Ausländer, S Cunna, S Linn, S Trowitzsch, S Trowitzsch, SC Almo, SE Luria, SJ Berríos-Rivera, SK Lee, SY Lee, T Maniatis, TC Terwilliger, VC Bode, W Abdulrahman, W Weber, WW Metcalf, Y Nie, Y Zhang   +71 more
core   +3 more sources

古細菌RNAリガーゼの機能解明研究 [PDF]

, 2022
筑波大学University of Tsukuba博士（人間生物学）Doctor of Philosophy in Human Biology2022doctoral ...
Liu, Yancheng, リウ, ヤンチェン, 劉, 雁成   +2 more
core   +1 more source

RXR Gamma Enables Oligodendrocyte Differentiation by Suppressing Sonic Hedgehog Signaling

Glia, Volume 74, Issue 6, June 2026.
Using pharmacogenetic approaches, Baldassarro et al. demonstrate that RXRγ suppresses the SHH signaling pathway in OPCs to ensure their efficient differentiation following T3 stimulation. Accordingly, Rxrg−/− OPCs displayed compromised differentiation which was normalized by the pharmacological inhibition of the hyperactive SHH pathway.
Vito Antonio Baldassarro, Quentin Brassart, Valérie Fraulob, Laura Calzà, Wojciech Krezel   +4 more
wiley   +1 more source

How cancer cells hijack DNA double-strand break repair pathways to gain genomic instability [PDF]

, 2015
DNA double-strand breaks (DSBs) are a significant threat to the viability of a normal cell, since they can result in loss of genetic material if mitosis or replication is attempted in their presence.
Arana, Audebert, Bartek, Bartkova, Barton, Bell, Bentley, Bentley, Beucher, Bolderson, Bouwman, Brady, Bryant, Budman, Budman, Bunting, Callen, Ceccaldi, Cerbinskaite, Chan, Chapman, Chapman, Choudhury, Cornforth, Deckbar, Deckbar, Deckbar, Dion, Dohrn, Ensminger, Erkko, Falck, Fan, Farmer, Fernandez-Capetillo, Fox, Fradet-Turcotte, Garcia, Gaymes, Gerhold, Geuting, Ghezraoui, Goodarzi, Helmink, Hu, Huertas, Jacot, Jasin, Jeggo, Jeggo, Jiang, Johnson, Kakarougkas, Kakarougkas, Kakarougkas, Kastan, Kemp, Kuhne, Li, Liang, Lieber, Lobrich, Loeb, Loucas, Maacke, Markus Löbrich, Mateos-Gomez, Michailidou, Muvarak, Neubauer, Panier, Panier, Penny A. Jeggo, Plisiecka-Halasa, Povirk, Prakash, Qiao, Radhakrishnan, Rahman, Riballo, Richardson, Roth, Sallmyr, Sarbajna, Seeber, Shanbhag, Shibata, Shibata, Shibata, Simsek, Soni, Steinel, Stephan, Syeda, Symington, Takenaka, Tepsuporn, Tobin, Tobin, Uziel, Wang, Wang, Ward, Waters, Waters, Williams, Woodbine, Woodbine, Yun, Zelensky, Zimmermann   +110 more
core   +1 more source