Results 111 to 120 of about 1,013 (157)
Histochemical Demonstration of Acid Inorganic Polyphosphatase. [PDF]
Journal of Histochemistry and Cytochemistry, 1955 A substrate solution for histochemical staining of acid inorganic polyphosphatase is prepared by controlled precipitation at pH 3.9, from ingredients totaling .014 M. lead, .05 M. acetate, .02 M. phosphate in the form of Graham's salt, and sufficient nitric acid to titrate.
George G Berg, G G Berg
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Properties of Polyphosphatase ofAcinetobacter johnsonii 210A
Antonie Van Leeuwenhoek, 1993 Polyphosphatase, an enzyme which hydrolyses highly polymeric polyphosphates to Pi, was purified 77-fold from Acinetobacter johnsonii 210A by Q-Sepharose, hydroxylapatite and Mono-Q column chromatography. The native molecular mass estimated by gel filtration and native gel electrophoresis was 55 kDa. SDS-polyacrylamide gel electrophoresis indicated that
Alexander Jb Zehnder, G J Kortstee
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Ppn2, A Novel Zn2+-Dependent Polyphosphatase in the Acidocalcisome-Like Yeast Vacuole [PDF]
Journal of Cell Science, 2017 ABSTRACT Acidocalcisome-like organelles are found in all kingdoms of life. Many of their functions, such as the accumulation and storage of metal ions, nitrogen and phosphate, the activation of blood clotting and inflammation, depend on the controlled synthesis and turnover of polyphosphate (polyP), a polymer of inorganic phosphate ...
Andreas Mayer, Rūta Gerasimaitė
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Purification and Properties of Polyphosphatase From Saccharomyces cerevisiae Cytosol
Yeast, 1998A homogenous polyphosphatase preparation was obtained from Saccharomyces cerevisiae cytosol with a 3.8% yield and 3540-fold purification. The enzyme hydrolysed polyphosphate (polyP) with various chain lengths, including polyP3, and split Pi off the end of the chain. It was inactive with respect to ATP, PPi, and p-nitrophenylphosphate.
Igor Sidorov +2 more
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Inorganic Polyphosphatase in the Developing Frog
Journal of Cellular and Comparative Physiology, 1955George G Berg, G G Berg
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Structural Analysis of a Multifunctional, Tandemly Repeated Inositol Polyphosphatase
Journal of Molecular Biology, 2009Mitsuokella multacida expresses a unique inositol polyphosphatase (PhyAmm) that is composed of tandem repeats (TRs). Each repeat possesses a protein tyrosine phosphatase (PTP) active-site signature sequence and fold. Using a combination of structural, mutational, and kinetic studies, we show that the N-terminal (D1) and C-terminal (D2) active sites of ...
Leonard Selinger
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The polyphosphatases of Aspergillus niger. II. Metabolic role of the polyphosphatases
Archives of Biochemistry and Biophysics, 1953Abstract 1. 1. The polyphosphatases of the mycelium of Aspergillus niger undergo characteristic changes in concentration depending upon cultural conditions, indicating that they are not mere structural units, but enter into active metabolism. 2. 2. There exists a definite relation between the specific polyphosphatase activity and the amount
P S, KRISHNAN, V, BAJAJ
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Survey of the Occurrence of Adenosine Polyphosphatase in Extracellular Matrix of Rat Tissues
Histochemistry, 1991The extracellular presence of adenosine polyphosphatase was investigated in a number of rat tissues. The enzyme was demonstrated in basement membranes of epithelial cells of duodenum, urinary bladder, tongue, choroid plexus, submandibular salivary gland, lung and kidney, as well as in basement membranes of capillaries in these tissues.
Klaas Poelstra +2 more
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