Results 171 to 180 of about 116,463,585 (197)

Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus

Journal of Virology, 1990
Purified virions or radiolabeled lysates of infected MA104 cells were used to characterize the structural and nonstructural polypeptides of a porcine group C rotavirus. At least six structural proteins were identified from purified group C rotavirus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
B M, Jiang, L J, Saif, S Y, Kang, J H, Kim   +3 more
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iTRAQ-based proteome analysis of porcine group A rotavirus-infected porcine IPEC-J2 intestinal epithelial cells

Journal of Proteomics, 2021
Porcine rotavirus (PoRV), particularly group A, is one of the most important swine pathogens, causing substantial economic losses in the animal husbandry industry. To improve understanding of host responses to PoRV infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography-tandem ...
Jinzhu, Zhou, Shimeng, Huang, Baochao, Fan, Beibei, Niu, Rongli, Guo, Jun, Gu, Song, Gao, Bin, Li   +7 more
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Comparative virulence of two porcine group-A rotavirus isolates in gnotobiotic pigs

American Journal of Veterinary Research, 1989
SUMMARY The virulence of 2 porcine group-A rotavirus isolates was compared. Forty hysterotomy-derived 3-day-old gnotobiotic pigs were inoculated orally with 2 ml of intestinal homogenate containing either the Ohio State University (OSU) or the South Dakota State University (SDSU) strain of porcine rotavirus or were inoculated with medium only. Clinical
J E, Collins, D A, Benfield, J R, Duimstra   +2 more
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Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity

Archives of Virology, 2006
A porcine Group A rotavirus strain (RU172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of Kolkata City, India. The G12 genotype specificity of RU172 was revealed by PCR-based genotyping assays following addition of a G12 type-specific primer (designed ...
S, Ghosh, V, Varghese, S, Samajdar, S K, Bhattacharya, N, Kobayashi, T N, Naik   +5 more
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Completion of the Four Large Gene Sequences of Porcine Group C Cowden Rotavirus

Virus Genes, 2000
The terminal nucleotide sequences of group C Cowden rotavirus gene segments 1-4 were determined. When compared with the published sequences, we found 14 to 29 additional nt at the 5' ends of the four reported gene sequences. For the 3' ends, we observed an additional 16 nt in gene 2 and 14 fewer nt in gene 4.
B, Jiang, L J, Saif, J R, Gentsch, R I, Glass   +3 more
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In vitro transcription and translation of genomic RNA from a porcine group C rotavirus

Archives of Virology, 1992
Eleven segments of ssRNA were synthesized from dsRNAs of a porcine group (Gp) C rotavirus (Cowden strain) using an in vitro transcription system. In vitro translation of unfractionated ssRNAs revealed at least nine viral proteins, ranging from 22 kDa to 93 kDa.
B M, Jiang, L J, Saif
openaire   +2 more sources

VP6 gene diversity in 11 Brazilian strains of porcine group C rotavirus

Virus Genes, 2014
Porcine group C rotavirus (RVC) is recognised as an enteric pathogen in piglets worldwide. The VP6 gene of RVC is divided into seven I-genotypes. Genotypes I2 and I3 are found in human and bovine strains, respectively; the porcine strains are divided into the other five genotypes (I1, I4-I7).
Danilo Tancler, Stipp, Alice Fernandes, Alfieri, Elis, Lorenzetti, Thais Neris, da Silva Medeiros, Flávia, Possatti, Amauri Alcindo, Alfieri   +5 more
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Production of OSU G5P[7] Porcine Rotavirus Expressing a Fluorescent Reporter via Reverse Genetics

Viruses
Rotaviruses are a significant cause of severe, potentially life-threatening gastroenteritis in infants and the young of many economically important animals.
John T Patton, Chantal Ama Agbemabiese
exaly   +2 more sources

Molecular analysis of the gene 6 from a porcine group C rotavirus that encodes the NS34 equivalent of group A rotaviruses

Virology, 1991
The complete nucleotide sequence of the gene 6 from the porcine group (Gp) C rotavirus strain Cowden was determined from gene 6-specific clones selected from a cDNA library and from viral transcript RNA. The gene is 1348 nucleotides in length with a potential initiation codon beginning at nucleotide 25 and a stop codon at nucleotide 1231.
Y A, Qian, B M, Jiang, L J, Saif, S Y, Kang, C K, Ojeh, K Y, Green   +5 more
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Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests

Journal of Clinical Microbiology, 1991
Three monoclonal antibodies (MAbs) to porcine group C rotavirus immunoprecipitated the major inner capsid protein (41 kDa) but failed to precipitate group A rotavirus proteins. In immunofluorescence tests of rotavirus-infected cell cultures or pig intestines, the MAbs recognized porcine and bovine group C rotaviruses but not group A or B rotaviruses ...
C K, Ojeh, B M, Jiang, H, Tsunemitsu, S Y, Kang, P A, Weilnau, L J, Saif   +5 more
openaire   +2 more sources