Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Benchmarking Illumina and Oxford Nanopore Technologies (ONT) sequencing platforms for whole genome sequencing of bacterial genomes and use in clinical microbiology. [PDF]
Purushothaman S +3 more
europepmc +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
High-resolution melting analysis for rapid and cost-effective detection of unnatural base pairs. [PDF]
van der Vlugt SEJ +4 more
europepmc +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Neurology of cognition and social behavior. A narrative review of neurobiological bases and clinical aspects. [PDF]
Campos-Sousa RN, Almeida KJ.
europepmc +1 more source
Synthesis, characterization, and anticancer potency of sulfadiazine salicylaldehyde-based Schiff bases. [PDF]
Nasr E +3 more
europepmc +1 more source
Nusinersen: the antisense oligonucleotide at the forefront of spinal muscular atrophy treatment. [PDF]
Singh NN, Ottesen EW, Singh RN.
europepmc +1 more source
Are you for real, <i>Tsukamurella</i>? Novel <i>Tsukamurella</i> species isolated from a patient with primary myelofibrosis. [PDF]
Lee J, Surette MD, Czech M, Zelazny AM.
europepmc +1 more source
RFID Positioning Based on Vehicle Positioning Subsystem
openaire +1 more source

