Results 131 to 140 of about 907 (167)

Expression of Human Cytomegalovirus pp150 Gene in Transgenic Vicia faba L. and Immunogenicity of pp150 Protein in Mice

Biologicals, 2010
The pp150 gene of human cytomegalovirus (HCMV) was transferred into Vicia faba plants by Agrobacterium tumefaciens-mediated transformation. Three of five hygromycin resistant V. faba plants were identified as positive by PCR and dot-blot hybridization. The ELISA results indicated that pp150 protein from three plants of transformed V.
Guocai Li, Weijuan Gong, Hongmei Jiao
exaly   +3 more sources

Prokaryotic Expression of Immunogenic Polypeptides of the Large Phosphoprotein (pp150) of Human Cytomegalovirus

Journal of General Virology, 1988
The large phosphorylated matrix protein pp150 of human cytomegalovirus (HCMV) is the polypeptide most frequently reactive in immunoblotting analyses with human antisera when compared with other viral proteins. Several defined regions of pp150 were expressed as beta-galactosidase fusion proteins and these were tested for their immunoreactivity with ...
Bettina Borisch, Michael Bröker, Gerhard Jahn   +2 more
exaly   +3 more sources

Nuclear Localization of the Human Cytomegalovirus Tegument Protein pp150 (ppUL32)

Journal of General Virology, 1995
The human cytomegalovirus (HCMV) basic phosphoprotein pp150, encoded by the UL32 gene, together with the two other major phosphoproteins, pp65 (ppUL83) and pp71 (ppUL82) and several minor structural proteins, form the tegument around the viral nucleocapsid.
Goetz Hensel, Sabine Gärtner
exaly   +3 more sources

Characterization of Host Immunity to Cytomegalovirus pp150 (UL32)

Human Immunology, 2005
The basic phosphoprotein 150 (pp150), the product of UL32 (unique long domain 32) gene of human cytomegalovirus (CMV), is an abundant component of the viral tegument and a target of human leukocyte antigen (HLA)-restricted cytotoxic T cells (CTLs) after infection.
Corinna La Rosa, Don J. Diamond, Simon F Lacey   +2 more
exaly   +3 more sources

Atomic Structure of the Human Cytomegalovirus Capsid With Its Securing Tegument Layer of pp150 [PDF]

Science, 2017
Strong under pressure Human cytomegalovirus (HCMV) is a member of the herpesvirus family that can cause life-threatening infections in those who are immunocompromised. HCMV encodes a genome that is about 50% larger than that of herpes simplex virus 1 (the virus that causes cold sores), but these two viruses have ...
Jonathan Jih, Jiansen Jiang, Xuekui Yu
exaly   +5 more sources

Control of Cytoplasmic Maturation Events by Cytomegalovirus Tegument Protein pp150. [PDF]

Journal of Virology, 2008
ABSTRACT Cytomegalovirus replication depends upon a betaherpesvirus-conserved 150-kDa tegument phosphoprotein (pp150; encoded by UL32) that supports the final steps in virion maturation at cytoplasmic assembly compartments. Amino acid substitutions were introduced into conserved region 1 (CR1) and CR2 of pp150, affecting a region
Ritesh Tandon, Edward S. Mocarski
exaly   +3 more sources

Cytotoxic T Lymphocyte (CTL) Responses to Human Cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in Healthy Individuals: Reevaluation of Prevalence of IE1-Specific CTLs.

Journal of Infectious Diseases, 2000
The prevalence of human cytomegalovirus (HCMV) pp65-, pp150-, IE1-exon4–, gB- and pp28-specific cytotoxic T lymphocyte (CTL) responses was compared among 34 healthy individuals, grouped by neutralizing antibody titers.Moderately and highly seropositive ...
Valéria Endrész, Katalin Burián, Stanley Plotkin   +2 more
exaly   +2 more sources

Human Cytomegalovirus Structural Proteins: Immune Reaction Against pp150 Synthetic Peptides. [PDF]

Journal of Clinical Microbiology, 1991
In the present study, several peptides of the major structural antigen (pp150) of human cytomegalovirus (CMV) have been chemically synthesized and tested by a modified slot blotting procedure for their ability to bind CMV-specific immunoglobulin G (IgG) and IgM present in human sera. The sequences of the peptides were deduced on the basis of either (i)
Maria Paola Landini, A Ripalti
exaly   +3 more sources

Bicaudal D1-Dependent Trafficking of Human Cytomegalovirus Tegument Protein pp150 in Virus-Infected Cells. [PDF]

Journal of Virology, 2010
ABSTRACT Human cytomegalovirus (HCMV) virion assembly takes place in the nucleus and cytoplasm of infected cells. The HCMV virion tegument protein pp150 (ppUL32) is an essential protein of HCMV and has been suggested to play a role in the cytoplasmic phase of HCMV assembly.
William J Britt, Mary E Ballestas
exaly   +3 more sources

Expression of Recombinant Human Cytomegalovirus Fusion Proteins pp150-pp65 Fragments and Its Application

Advanced Materials Research, 2013
Objective To evaluate the effectiveness of prokaryotic expression of fusion proteins pp150-pp65 of human cytomegalovirus (hCMV) for its application as antigen, the fusion protein of pp150-pp65 were expressed in prokaryotic expression system and purified by Ni-NTA affinity chromatography column for preparing the colloidal gold kit.
Hua Yan, Ting Yu
exaly   +2 more sources