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Detection of cytomegalovirus antibodies by an enzyme-linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150 [PDF]
Journal of Clinical Microbiology, 1992 Parts of the large phosphorylated tegument protein, pp150, of human cytomegalovirus (HCMV) were expressed in bacteria. The resulting fusion proteins were tested in a Western blot (immunoblot) assay for reactivity with a monoclonal antibody against pp150, with a polyspecific rabbit antiserum, and with human reconvalescent-phase sera.
Bodo Plachter, G Jahn
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Virus Research, 1989
An HCMV specific clone was isolated from a genomic library of human cytomegalovirus (HCMV) DNA cloned into the expression vector lambda gt11. This clone (lambda 111-1) expressed an HCMV/beta-galactosidase fusion protein which was reactive with rabbit antibody prepared against purified HCMV virions and dense bodies as well as human HCMV immune serum. By
H, Pande +3 more
openaire +2 more sources
An HCMV specific clone was isolated from a genomic library of human cytomegalovirus (HCMV) DNA cloned into the expression vector lambda gt11. This clone (lambda 111-1) expressed an HCMV/beta-galactosidase fusion protein which was reactive with rabbit antibody prepared against purified HCMV virions and dense bodies as well as human HCMV immune serum. By
H, Pande +3 more
openaire +2 more sources
[Predication of secondary structures and epitopes of fusion protein pp150/MDBP].
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2009The secondary structures of fusion protein pp150/MDBP, including alpha-helix, beta-sheet, turn regions, were analyzed by Garnier-Robson's and Chou-Fasman's methods; the antigenic epitopes of B cells were analysed by using hydrophilicity plot. The results showed that the fusion protein pp150/MDBP might have less alpha-helix, but be rich in beta-sheet ...
Dadong, Guo, Xueqin, Gao, Jinxiang, Han
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010
Human cytomegalovirus (HCMV) infection is an ubiquitous herpesvirus disease in human populations. It is rarely pathogenic to healthy adults, yet it may cause severe outcome to organ transplant recipients, the immunocompromised individuals and pregnant women. Using DNA from HCMV AD169 strain as template, the UL32 gene encoding pp150 protein fragment and
Da-Dong, Guo +4 more
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Human cytomegalovirus (HCMV) infection is an ubiquitous herpesvirus disease in human populations. It is rarely pathogenic to healthy adults, yet it may cause severe outcome to organ transplant recipients, the immunocompromised individuals and pregnant women. Using DNA from HCMV AD169 strain as template, the UL32 gene encoding pp150 protein fragment and
Da-Dong, Guo +4 more
openaire +1 more source
[Study on immunogenicity of DNA vaccine encoding human cytomegalovirus gB and pp150 fusion gene].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 2011To provide experimental evidence for development of human cytomegalovirus (HCMV) nucleic acid vaccine, HCMV surface protein (gB), membrane protein (pp150), and gB-pp150 fused gene eukaryotic expression vector were constructed.gB and pp150 genes were amplified and fused into gB-pp150, then were cloned into pcDNA 3.1 (+) to obtain recombinant expression ...
Wen-Yuan, Shi +4 more
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Molekuliarnaia genetika, mikrobiologiia i virusologiia, 1996
The epidemiological situation with cytomegalovirus infection in Siberia is still to be studied and serological diagnosis of human cytomegalovirus (HCMV) is not satisfactory. Two regions of HCMV genome (strain AD-169) have been examined for obtaining diagnostic reagents by expression cloning.
M A, Susloparov +7 more
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The epidemiological situation with cytomegalovirus infection in Siberia is still to be studied and serological diagnosis of human cytomegalovirus (HCMV) is not satisfactory. Two regions of HCMV genome (strain AD-169) have been examined for obtaining diagnostic reagents by expression cloning.
M A, Susloparov +7 more
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PP150-SUN BIOELECTRICAL IMPEDANCE AND PHASE ANGLE: HOW RELEVANT IN CANCER?
Clinical Nutrition Supplements, 2012P. Ravasco +4 more
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SUN-PP150: Nutrient Deficiency in Hemodialysis Patients even after Adjusting for Underreporters
Clinical Nutrition, 2015A. Moreira, F. Domingos, A. Gaspar
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PP150-MON: Energy Requirements in Obese Patients with Low Activities of Daily Living
Clinical Nutrition, 2014H. Asakura +4 more
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