Results 141 to 150 of about 2,366 (165)

Expression of human cytomegalovirus pp150 gene in transgenic Vicia faba L. and immunogenicity of pp150 protein in mice

Biologicals, 2010
The pp150 gene of human cytomegalovirus (HCMV) was transferred into Vicia faba plants by Agrobacterium tumefaciens-mediated transformation. Three of five hygromycin resistant V. faba plants were identified as positive by PCR and dot-blot hybridization. The ELISA results indicated that pp150 protein from three plants of transformed V.
Hua, Yan, Huishen, Yan, Guocai, Li, Weijuan, Gong, Hongmei, Jiao, Hongju, Chen, Mingchun, Ji   +6 more
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Characterization of Host Immunity to cytomegalovirus pp150 (UL32)

Human Immunology, 2005
The basic phosphoprotein 150 (pp150), the product of UL32 (unique long domain 32) gene of human cytomegalovirus (CMV), is an abundant component of the viral tegument and a target of human leukocyte antigen (HLA)-restricted cytotoxic T cells (CTLs) after infection.
Corinna, La Rosa, Zhongde, Wang, Simon F, Lacey, Susan F, Markel, Madeva C, Sharma, Joybelle, Martinez, Maria M, Lalimarmo, Don J, Diamond   +7 more
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PP150 Bevan Health Technology Exemplars: Early Dialogue To Systematize HTA

International Journal of Technology Assessment in Health Care, 2019
IntroductionWales has ambitious health, wealth, and innovation policies and a clear goal to use the economic muscle of the Welsh National Health Service (NHS) to support its strong life sciences sector. Health Technology Wales (HTW) has a clear remit to appraise technologies over the span of their lifecycle from innovation to obsolescence.
Susan Myles, Sion Charles, Claire Davies, Lauren Elston, Helen Howson, Ruth Louise Poole   +5 more
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PP150 Rapid Analgesia For Prehospital Hip Disruption: A Feasibility Study

International Journal of Technology Assessment in Health Care, 2017
INTRODUCTION:Adequate pain relief at the scene of injury and during transport to hospital is a major challenge in all acute traumas, especially for those with hip fractures, whose injuries are difficult to immobilize and long-term outcomes may be adversely affected by administration of opiate analgesics.
Jenna Bulger, Alan Brown, Bridie Evans, Greg Fegan, Simon Ford, Katy Guy, Sian Jones, Leigh Keen, Ashrafunnesa Khanom, Ian Pallister, Nigel Rees, Ian Russell, Anne Seagrove, Helen Snooks   +13 more
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PP150. Pregnancy outcome in women with heart disease in a single tertiary care center

Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 2012
The number of women presenting with cardiac disease during pregnancy is increasing because more women with repaired congenital heart disease are reaching childbearing age and because of more pregnancies occurring in women with ischemic heart disease. Cardiac disease is the leading cause of indirect maternal mortality in the Netherlands.Clinical data of
E, Hink, S H, Luitjes, A C, Bolte
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Expression of Recombinant Human Cytomegalovirus Fusion Proteins pp150-pp65 Fragments and its Application

Advanced Materials Research, 2013
Objective To evaluate the effectiveness of prokaryotic expression of fusion proteins pp150-pp65 of human cytomegalovirus (hCMV) for its application as antigen, the fusion protein of pp150-pp65 were expressed in prokaryotic expression system and purified by Ni-NTA affinity chromatography column for preparing the colloidal gold kit.
Yu Fen Jin, Yan Lei Li, Yan Hua, Xiao Gang Zhang, Ting Yu   +4 more
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Genomic locus for a 140 kDa structural protein (pp150) of human cytomegalovirus in strains Towne and AD169

Virus Research, 1989
An HCMV specific clone was isolated from a genomic library of human cytomegalovirus (HCMV) DNA cloned into the expression vector lambda gt11. This clone (lambda 111-1) expressed an HCMV/beta-galactosidase fusion protein which was reactive with rabbit antibody prepared against purified HCMV virions and dense bodies as well as human HCMV immune serum. By
H, Pande, K, Campo, M A, Churchill, J A, Zaia   +3 more
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Establishment and validation of an enzyme-linked immunosorbent assay for IgG antibody against cytomegalovirus based on pp150 antigen

Journal of Virological Methods, 2017
The development of HCMV vaccines for the prevention of congenital HCMV has been identified as a top priority by the Institute of Medicine (USA), and virus infection is an important endpoint for the efficacy evaluation of the candidate vaccines. However, it is technically difficult to capture the HCMV viremia or uremia in infected individuals because ...
Huang, Xi, Li, Jinjie, Ge, Shengxiang, Li, Tingdong, Wang, Han, Guo, Xiaoyi, Fu, Tong-Ming, Zhang, Jun   +7 more
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[Predication of secondary structures and epitopes of fusion protein pp150/MDBP].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2009
The secondary structures of fusion protein pp150/MDBP, including alpha-helix, beta-sheet, turn regions, were analyzed by Garnier-Robson's and Chou-Fasman's methods; the antigenic epitopes of B cells were analysed by using hydrophilicity plot. The results showed that the fusion protein pp150/MDBP might have less alpha-helix, but be rich in beta-sheet ...
Dadong, Guo, Xueqin, Gao, Jinxiang, Han
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[Expression of recombinant human cytomegalovirus fusion proteins pp150/MDBP fragments and its application].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010
Human cytomegalovirus (HCMV) infection is an ubiquitous herpesvirus disease in human populations. It is rarely pathogenic to healthy adults, yet it may cause severe outcome to organ transplant recipients, the immunocompromised individuals and pregnant women. Using DNA from HCMV AD169 strain as template, the UL32 gene encoding pp150 protein fragment and
Da-Dong, Guo, Xue-Qin, Gao, Jin-Xiang, Han, Yi, Liu, Hua-Ning, Zhang   +4 more
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