Results 21 to 30 of about 4,915 (175)

Cryopreservation and characterization of canine preantral follicles

Cryobiology, 2018
The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,
J.L.P.R. Jivago, F. Paulini, R.C. Silva, M.S. Araujo, A.P.S. Marinho, C.M. Lucci   +5 more
openaire   +2 more sources

In Vitro Growth of Preantral Follicle and Maturation of Intrafollicular Oocyte from Aged Mice

Journal of Animal Reproduction and Biotechnology, 2019
This study aimed to recover the ovarian function through in vitro culture of preantral follicles from aged mice. First, we isolated the preantral follicles from ovaries of sixty-seven-week old B6D2F1 mice with decreased fecundity to know how many ...
Jung-Ah Yoon, Jung-Kyu Choi
doaj   +1 more source

Ovarian features in white-tailed deer (Odocoileus virginianus) fawns and does.

PLoS ONE, 2017
The knowledge about ovarian reserve is essential to determine the reproductive potential and to improve the methods of fertility control for overpopulated species, such as white-tailed deer (Odocoileus virginianus). The goal of this study was to evaluate
G D A Gastal, A Hamilton, B G Alves, S G S de Tarso, J M Feugang, W J Banz, G A Apgar, C K Nielsen, E L Gastal   +8 more
doaj   +1 more source

Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Nature Communications, 2022
Vitrification of preantral follicles is a promising strategy for female fertility preservation, but requires high concentrations of toxic cryoprotectants. Here, the authors accomplish follicle vitrification with low cryoprotectants by taking advantage of
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao   +5 more
doaj   +1 more source

Cryopreservation of Preantral Follicles

, 2018
In mammals, the total number of female germ cells is already established by the time of birth, meaning that no mitosis will take place in oogonias thereafter. Their cryostorage, therefore, depends on ovarian tissue manipulation. As an alternative to mature oocyte cryopreservation, the maintenance of inactive preantral follicles is a remarkable option ...
Rivas Leonel, Ellen Cristina, Lucci, Carolina Madeira, Andrade Amorim, Christiani   +2 more
openaire   +4 more sources

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Reproduction in Domestic Animals, 2013
ContentsThe aim of this study was to test the use of mechanical and mechanical‐enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase).
Rivas Leonel, Ellen, Bento‐Silva, V, Ambrozio, KS, Luna, HS, Costa e Silva, EV, Zúccari, CESN   +5 more
openaire   +3 more sources

Activin promotes oocyte development in ovine preantral follicles in vitro

Reproductive Biology and Endocrinology, 2003
Activins have been implicated as important regulating factors for many reproductive processes. The aim of this study was to determine the effect of activin A on the development of ovine preantral follicles in vitro.
Telfer Evelyn E, Armstrong David G, Thomas Fiona H   +2 more
doaj   +1 more source

In vitro culture of bovine preantral follicles: a review [PDF]

Reproductive Biology and Endocrinology, 2014
Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular ...
Araújo, Valdevane R, Gastal, Melba O, Figueiredo, José R, Gastal, Eduardo L   +3 more
openaire   +2 more sources

Morphometric and ultrastructural characterization of Bos indicus preantral follicles [PDF]

Animal Reproduction Science, 2005
The aim of the present study was to characterize the ultrastructure of zebu cow preantral follicles (PAFs). Ovarian cortex samples were processed for light and transmission electron microscopy. Primordial follicles consisted of an oocyte surrounded by one layer of flattened or flattened-cuboidal granulosa cells. The oocyte contained a large and usually
Kacinskis, Mirella Ávila, Lucci, Carolina Madeira, Luque, Maria Carolina Aquino, Báo, Sônia Nair   +3 more
openaire   +2 more sources

Effects of sodium alginate capsules as 3D scaffolds on hormones and genes expression in preantral follicles of mice compared to 2D medium: An experimental study

International Journal of Reproductive BioMedicine, 2020
Background: The improvement of in vitro maturation methods, which can activate the preantral follicle growth, plays a crucial role in the production of mature oocytes in reproductive technology.
Cyrus Jalili, Fuzieh Khani Hemmatabadi, Kamran Mansouri, Mehrdad Bakhtiyari   +3 more
doaj   +1 more source