Results 91 to 100 of about 5,818,450 (268)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Real-Time Precise Point Positioning Method Considering Broadcast Ephemeris Discontinuities

Navigation
With advancements in the broadcast ephemeris accuracy of global navigation satellite systems (GNSSs), precise point positioning based on broadcast ephemeris (BE-PPP) is gradually showing promising prospects.
Quanrun Cheng, Junping Chen, Yize Zhang, Chao Yu   +3 more
doaj   +1 more source

A Decentralized Processing Schema for Efficient and Robust Real-time Multi-GNSS Satellite Clock Estimation

Remote Sensing, 2019
Real-time multi-GNSS precise point positioning (PPP) requires the support of high-rate satellite clock corrections. Due to the large number of ambiguity parameters, it is difficult to update clocks at high frequency in real-time for a large reference ...
Xinyuan Jiang, Shengfeng Gu, Pan Li, Maorong Ge, Harald Schuh   +4 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Performance of Selected Ionospheric Models in Multi-Global Navigation Satellite System Single-Frequency Positioning over China

Remote Sensing, 2019
Ionospheric delay as the major error source needs to be properly handled in multi-GNSS (Global Navigation Satellite System) single-frequency positioning and the different ionospheric models exhibit apparent performance difference.
Ahao Wang, Junping Chen, Yize Zhang, Lingdong Meng, Jiexian Wang   +4 more
doaj   +1 more source

Developing regional precise positioning services using the legacy and future GNSS receivers [PDF]

, 2009
This paper presents an overview of technical solutions for regional area precise GNSS positioning services such as in Queensland. The research focuses on the technical and business issues that currently constrain GPS-based local area Real Time Kinematic (
Tang, Maolin, Higgins, Matt, Rizos, Chris, Feng, Yanming, Lim, Samsung   +4 more
core  

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source