Results 191 to 200 of about 101,629 (318)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Enhanced precise point positioning for GNSS users

open access: yes
This paper summarizes the main results obtained during the development of an Enhanced Precise Point Positioning (EPPP) Global Navigation Satellite Systems multifrequency user algorithm.
Samson, Jaron   +11 more
core  

Precise Point Positioning under Degraded Forest Conditions

open access: yes, 2014
Denne oppgaven tar utgangspunkt i Precise Point Positioning under degraderte skogstettheter. Gjennom beregning av sikt på bakgrunn av elevasjonsvinkler innmålt i feltarbeid, innmåling av posisjonsdata og beregninger gjort med etterprosesseringstjenestene
Jensen, Andreas Saxi
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Time correlation in GNSS precise point positioning

open access: yes, 2013
Precise point positioning (PPP) is a GNSS processing technique that utilizes undifferenced observations to calculate a position to centimeter accuracy.
Goode MED   +3 more
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

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