Results 91 to 100 of about 1,305,221 (200)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Alimentos ultraprocesados: evaluación de su clasificación, consumo y huellas epigenéticas asociadas

Revista Española de Nutrición Humana y Dietética, 2021
Celia Martinez-Perez, Rodrigo San-Cristobal, Cristina Climent-Mainar, Lidia Daimiel, José M Ordovás   +4 more
doaj  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Estrategia de muestreo para la estimación de la tasa de favoritismo en la elección presidencial

Revista Colombiana de Estadística, 2005
Se fijan los objetivos y se definen los criterios metodológicos de una Encuesta Nacional de Favoritismo en Elecciones Presidenciales. Utilizando el hecho de que el candidato H. Serpa se presentó como candidato en 1998 y en 2002 se utilizan los resultados
LEONARDO BAUTISTA SIERRA
doaj  

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Validity and Reliability of an Inertial Device (WIMU PROTM) to Tracking Analysis in Handball

E-balonmano.com: Revista de Ciencias del Deporte, 2018
Thanks to technological advances, new methods to analyse physiological, kinematical and spatial positioning variables have been developed. The collection of quantitative data will enable the training process enhancement in sport.
Alejandro Bastida-Castillo, Carlos David Gómez-Carmona, Alejandro Hernández-Belmonte, José Pino-Ortega   +3 more
doaj  

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Ruminant feces used as inoculum for the in vitro gas production technique Heces de rumiantes como fuente de inóculo para la técnica in vitro de producción de gases Fezes de ruminantes como fonte de inoculo para a técnica in vitro de produção de gases

Revista Colombiana de Ciencias Pecuarias, 2012
Background: ruminal feed fermentation can be studied through in vitro gas production. However, this technique requires fistulated animals from which to obtain the inoculum, which limits its use. Objective: the objective of this experiment was to evaluate
Sandra L Posada, Ricardo R Noguera, Juan A Segura   +2 more
doaj  

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source