Results 81 to 90 of about 114,348 (307)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

SmartPlus: a computer-based image analysis method to predict continuous-valued vascular abnormality index in Retinopathy of Prematurity

International Journal of Retina and Vitreous
Plus disease is characterized by abnormal changes in retinal vasculature of premature infants. Presence of Plus disease is an important criterion for identifying treatment-requiring cases in Retinopathy of Prematurity (ROP).
Sayed Mehran Sharafi, Nazanin Ebrahimiadib, Ramak Roohipourmoallai, Afsar Dastjani Farahani, Marjan Imani Fooladi, Golnaz Gharehbaghi, Elias Khalili Pour   +6 more
doaj   +1 more source

Optical Coherence Tomography and Wide-Field Fluorescein Angiography in Retinopathy of Prematurity

, 2017
The advent and adaptation of many imaging modalities promise to revolutionize our understanding of retinopathy of prematurity (ROP) by improving the detection, diagnosis, and monitoring of response to treatment of this disease.
Cynthia A. Toth, Lejla Vajzovic, Maldonado, Ramiro S., Rothman, Adam L., Ramiro S. Maldonado, Vajzovic, Lejla, Adam L. Rothman, Toth, Cynthia A.   +7 more
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Factors associated with delayed vascular maturation in infants with retinopathy of prematurity not requiring treatment

Çukurova Üniversitesi Tıp Fakültesi Dergisi, 2016
Purpose: The aim of this study is to determine risk factors associated with delayed regression of retinopathy of prematurity and prolonged vascular completion in patients not requiring treatment.
Ebru Esen, Selcuk Sizmaz, Hacer Yapicioglu, Ferda Ozlu, Kemal Yar, Ilker Unal, Nihal Demircan   +6 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Early applicability of retinopathy of prematurity score (ROPScore) and serum insulin growth factor-1 (IGF-1) for predicting treatment-requiring retinopathy of prematurity: a prospective cohort study

Egyptian Pediatric Association Gazette
Background Retinopathy of prematurity is a leading cause of blindness especially in developing countries where the accessibility to screening may not be available.
Aliaa Adel Ali, Nancy Abd El-Salam Ahmed Gomaa, Basma Samir Kamil, Ahmed Awadein, Heba Baz, Esraa Ahmed Elmazzahy   +5 more
doaj   +1 more source

Toward a Modification of the Indices of Neonatal Prematurity

, 1975
In a study of 233 infants, traditional indices of neonatal prematurity were excessively high for predicting developmental lag at 1 yr., and alternative cut-off scores were suggested.
Daniel V. Caputo, Harvey B. Taub, Kenneth M. Goldstein   +2 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source