Results 11 to 20 of about 1,409,327 (243)
, 2014 Primers used for real-time PCR against cell cultured isolate and field strains of ASCV and primers used in virus genome cloning and sequencing.*Nucleotides matching cell cultured isolate only are underlined.Primers.
Karine Lindmo (268077) +6 more
core +5 more sources
, 2022 Y’-element forward (Y’F) and reverse (Y’R) primers were used as 32P end-labeled single strand DNA probes (Fig 1A). Four X-element primers (‘a’ to ‘d’) were used in the Taq polymerase primer extension assay (Fig 1B).
Brian Luke (133715) +5 more
core +2 more sources
MCMC-ODPR : primer design optimization using Markov Chain Monte Carlo sampling [PDF]
, 2012 Background Next generation sequencing technologies often require numerous primer designs that require good target coverage that can be financially costly.
Kitchen, James +7 more
core +1 more source
Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis [PDF]
, 1995 Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating ...
O'Donoghue, P. +17 more
core +1 more source
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J. +27 more
core +2 more sources
Primer1: Primer design Web service for Tetra-Primer ARMS-PCR [PDF]
, 2012 Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of ...
Ke, Xiayi, Collins, Andrew
core +1 more source
Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology
Plants, 2020 In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine,
Stefano Panno +7 more
doaj +1 more source
Alternatives in Molecular Diagnostics of Encephalitozoon and Enterocytozoon Infections
Journal of Fungi, 2020 Microsporidia are obligate intracellular pathogens that are currently considered to be most directly aligned with fungi. These fungal-related microbes cause infections in every major group of animals, both vertebrate and invertebrate, and more recently ...
Alexandra Valenčáková, Monika Sučik
doaj +1 more source