Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies [PDF]
Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually ...
Tomasz Bogiel +2 more
doaj +2 more sources
Biotyping of isolates of Pseudomonas aeruginosa isolated from human infections by RAPD and ERIC-PCR
Pseudomonas aeruginosa is a significant mortality factor due to nosocomial infections in humans. P. aeruginosa has been known with severe infections, high incidence, and multiple drug resistance. The present study aims to rapidly diagnose and biotype the
Aida Hematzadeh, Masoud Haghkhah
doaj +3 more sources
Several methodologies in molecular biology have been used in theinvestigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and
Patrícia da Silva Nascente +5 more
doaj +2 more sources
Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran [PDF]
Background: Multidrug resistant K. pneumoniae, particularly the extended-spectrum β-lactamase (ESBL) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. Employing molecular methods to distinguish
Fereshteh Eftekhar, Parvaneh Nouri
doaj +2 more sources
Parentage analysis using RAPD PCR
M P, Scott, K M, Haymes, S M, Williams
exaly +4 more sources
Antibiotic Resistance Pattern and Molecular Typing by PCR-RAPD Analysis in Clinical Isolates of Pseudomonas aeruginosa from Motahari Hospital, Tehran, Iran [PDF]
Background: Pseudomonas aeruginosa infection is one of the major challenges in burn patients. This organism is resistant to a wide range of antibiotics.
Mahmood Barati +5 more
doaj +1 more source
Background: In this study, it was aimed to investigate Mycobacterium bovis strains isolated from lungs and lymph nodes of slaughtered animals on clonal level by using different methods such as spoligotyping, enterobacterial repetitive intergenic ...
Funda Sahin +4 more
doaj +1 more source
Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) [PDF]
DNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random amplified polymorphic DNA (RAPD) assay is a useful method for detecting genotoxin-induced DNA damage.
Ercan Fahriye Sumer
doaj +1 more source
Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR
Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is ...
INGRID GEORGINA ORCE +5 more
doaj +1 more source
Objective Species identification of Shigella isolates are so prominent for epidemiological studies and infection prevention strategies. We developed and evaluated RAPD and ERIC-PCR coupled with HRM for differentiation of non-dysenteriae Shigella species ...
Babak Pakbin +5 more
doaj +1 more source

