Results 91 to 100 of about 388,989 (251)

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

A Bioreactor-Based Yellow Fever Virus-like Particle Production Process with Integrated Process Analytical Technology Based on Transient Transfection. [PDF]

Viruses, 2023
Dekevic G, Tertel T, Tasto L, Schmidt D, Giebel B, Czermak P, Salzig D.   +6 more
europepmc   +1 more source

Hyperspectral Imaging For Process Analytical Technology

, 2011
Hyperspectral Imaging (HI) is a valuable tool for online-analysis and control of production processes. HI is an emerging platform technology that integrates spectroscopy and imaging to gain both spectral and spatial information from a sample. The combination with Multivariate Data Analysis (MVA) permits extraction of the relevant information.
Ostertag, Edwin, Kessler, Rudolf W.
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Machine learning and metabolic modelling assisted implementation of a novel process analytical technology in cell and gene therapy manufacturing. [PDF]

Sci Rep, 2023
Williams T, Kalinka K, Sanches R, Blanchard-Emmerson G, Watts S, Davies L, Knevelman C, McCloskey L, Jones P, Mitrophanous K, Miskin J, Dikicioglu D.   +11 more
europepmc   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Unveiling the Metabolomic, Phytochemical and Bioactive Profile of Twelve Macroalgae from the Adriatic Sea: A Comprehensive Analysis Using MSPD-UHPLC-QTOF

Phycology
The present study provides an exhaustive exploration of twelve macroalgal species from the Adriatic Sea, including seven brown algae (Ericaria amentacea, Fucus virsoides, Cutleria multifida, Cystoseira compressa, Cystoseira corniculata, Gongolaria ...
Aly Castillo, María Celeiro, Marta Lores, Kristina Perišić, Krunoslav Aladić, Stela Jokić   +5 more
doaj   +1 more source

Real-time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process. [PDF]

Eng Life Sci, 2022
Wang X, Tian Y, Zhang S, Qu H.
europepmc   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source