Results 101 to 110 of about 21,427,686 (296)

Spatially and Temporally Resolved Analysis of Bleeding in a Centrifugal Partition Chromatography Rotor

open access: yesSeparations
Centrifugal Partition Chromatography (CPC) is a separation technique that utilizes immiscible liquid phases to purify compounds. The selection of solvents in Liquid–Liquid Chromatography offers flexibility and optimization possibilities for specific ...
Felix Buthmann   +4 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

open access: yesFEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz   +11 more
wiley   +1 more source

Enhanced Protein Recovery from Rapeseed Press Cake via Pressurized Liquid Extraction: Effects of pH Shifting and Process Parameters

open access: yesApplied Sciences
Rapeseed press cake (RPC), the protein-rich residue from edible oil production, is currently underutilized and is primarily used as animal feed. This study aimed to investigate pressurized liquid extraction (PLE) for protein recovery from RPC using ...
Christoforos Vasileiou   +4 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

open access: yesFEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure   +3 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

open access: yesFEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

First-principle modeling of parallel-flow regenerative kilns and their optimization with genetic algorithm and gradient-based method

open access: yesDigital Chemical Engineering
We present a one-dimensional first-principle model for parallel-flow regenerative kilns that accounts for the most important effects. These include the kinetics and thermal effects of the limestone decomposition as well as the heat transfer between the ...
Michael Kreitmeir   +4 more
doaj   +1 more source

The Design Process Explained: Video 1 Introduction

open access: yes, 2019
This video provides an introduction to the Double Diamond Design Process and provides an overview of the whole video series, showing brief examples from each stage of the design process and why it is ...
Charlie Ranscombe, Kate Bissett-Johnson
core  

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

open access: yesFEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa   +2 more
wiley   +1 more source

Project Process Cards: A Self Evaluation Tool for Design Studio

open access: yes, 2019
Industrial design education is about process rather than product. Design education requires students to follow particular paths for learning design practice during their journey to proposing design solutions.
Öztoprak, Aydın   +5 more
core   +1 more source

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