Results 181 to 190 of about 55,466 (262)

Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations. [PDF]

Biotechnol Prog
Altenburg JJ, Juarez-Garza BE, van Keimpema J, van Oosten L, Pijlman GP, van Oers MM, Wijffels RH, Martens DE.   +7 more
europepmc   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Corrigendum to "A new reactor for process intensification involving the simultaneous application of adjustable ultrasound and microwave radiation" [Ultrason. Sonochem. 77 (2021) 105701]. [PDF]

Ultrason Sonochem
Calinescu I, Vinatoru M, Ghimpețeanu D, Lavric V, Masom TJ, Vartolomei A.   +5 more
europepmc   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Process intensification of pharmaceutical powder blending at commercial throughputs by utilizing semi-continuous mini-blending. [PDF]

Int J Pharm X
Jaspers M, Tegel F, Roelofs TP, Starsich F, Song YL, Meir B, Elkes R, Dickhoff BHJ.   +7 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Selective Hydrogenation of Quercetin to Dihydroquercetin Using a Carbon Nanotube-Supported Non-Noble Nickel Catalyst

ACS Omega
Yujie He, Hairong Mo, Chenghong Wu, Xu Wei, Xiaopeng Chen, Jiezhen Liang, Xiaojie Wei, Linlin Wang   +7 more
doaj   +1 more source

Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification. [PDF]

Nat Commun
Zheng D, Zheng Y, Tan J, Zhang Z, Huang H, Chen Y.   +5 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Effects of process intensification on homogeneity of an IgG1:κ monoclonal antibody during perfusion culture. [PDF]

Appl Microbiol Biotechnol
Liang G, Madhavarao CN, Morris C, O'Connor T, Ashraf M, Yoon S.   +5 more
europepmc   +1 more source