Results 191 to 200 of about 145,574 (266)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The evaluation of motor deficits in children with ulcerative colitis: a cross-sectional study. [PDF]

Eur J Pediatr
Akbulut UE, Atalay A, Isik İA, Sivil N, Sarı M, Birsen G, Kara OK, Kara K, Ulu HS, İnal HA.   +9 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

10-yr Outcomes of Senior Clinical Fellowship in Robot-assisted Radical Cystectomy (RARC) with focus on Intracorporeal Ileal Conduit Urinary Diversion: Aggregated Learning Curve Assessment and Achievement of the EAU-Robotic Urology Section (ERUS) RARC Curriculum. [PDF]

Eur Urol Open Sci
Del Giudice F, Santarelli V, Spurna K, Kirmani SGA, Bhatti NUH, Gad M, Asero V, Chung BI, Crocetto F, Challacombe B, Abu-Ghanem Y, Mensah E, Ibrahim Y, Chatterton K, Amery S, Alao R, O'Kelly J, Parsons BA, Tan TW, Moran D, Doerge Unger E, Rai S, Furrer MA, Kam JS, Nair R, Sahai A, Dasgupta P, Thurairaja R, Khan MS.   +28 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

One test, many tongues: Surveying language proficiency across the globe. [PDF]

Proc Natl Acad Sci U S A
van Rijn P, Sun Y, Lee H, Marjieh R, Sucholutsky I, Lanzarini F, André E, Jacoby N.   +7 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Parent-child mental health literacy discordance and adolescent depression and anxiety: a population-based cross-sectional study. [PDF]

Child Adolesc Psychiatry Ment Health
Zhong SL, Hou CL, Jia FJ, Wang SB, Zhou L.   +4 more
europepmc   +1 more source