Extensive evolution of cereal ribosome-inactivating proteins translates into unique structural features, activation mechanisms, and physiological roles [PDF]
, 2017 Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can depurinate rRNAs thereby inhibiting protein translation. Although these proteins have also been detected in bacteria, fungi, and even some insects, they are especially ...
De Zaeytijd, Jeroen, Van Damme, Els
core +2 more sources
Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells. [PDF]
Adv Pharm Bull, 2023 Alizadeh AA +4 more
europepmc +1 more source
Advanced Science, EarlyView.This study proposes a universal catalytic DNA circuits termed TACTIC (Thermus thermophilus protein‐driven autocatalytic circuit) for one‐pot detection of DNA and RNA in multiple clinical samples. Integrated with machine learning, TACTIC accurately profile the distinct expression of four extracellular vesicle‐derived miRNAs across different samples and ...
Zuowei Xie +12 more
wiley +1 more source
Who Is the Intermediate Host of RNA Viruses? A Study Focusing on SARS-CoV-2 and Poliovirus
MicroorganismsThe COVID-19 pandemic has sparked a surge in research on microbiology and virology, shedding light on overlooked aspects such as the infection of bacteria by RNA virions in the animal microbiome.
Carlo Brogna +9 more
doaj +1 more source
Nature Communications, 2020 A key feature that differentiates prokaryotic cells from eukaryotes is the absence of an intracellular membrane surrounding the chromosomal DNA. Here, the authors isolate a member of the ubiquitous, yet-to-be-cultivated bacterial phylum ‘Candidatus ...
Taiki Katayama +8 more
doaj +1 more source
A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation. [PDF]
Plant Methods, 2023 Wu JR +5 more
europepmc +1 more source
Mimicking Life: Autonomous Oscillating Artificial Cilia Driven by Chemical Power
Advanced Intelligent Systems, EarlyView.The synthesis and motion analysis of chemically actuated, individually autonomous artificial cilia are presented. Driven by an internal chemical reaction, the self‐driven individual cilia require no external stimuli. They undergo periodic oscillatory motion with a 3D beat pattern and exhibit chemotactic shifts, reminiscent of biological systems.
Rajata Suvra Chakrovorty +2 more
wiley +1 more source
Virus production in shallow groundwater at the bank of the Danube River.
PLoS ONEViruses play a crucial role in regulating prokaryotic populations, yet their impact on subsurface environments, specifically groundwater habitats, remains poorly understood.
Daniel Pleyer +2 more
doaj +1 more source
Insights into organelle forming RNAs: Diversity, functions and future perspectives
Animal Models and Experimental Medicine, EarlyView.RNA molecules play crucial roles in the formation and maintenance of cellular structures and organelles. These ‘organelle formation RNAs’ include ribosomal RNAs, paraspeckle‐forming RNAs, nuclear speckle‐forming RNAs, nucleolus‐forming RNAs, and cytoskeleton‐forming RNA.
Meng Gong, Xiangting Wang, Xiaolin Liang
wiley +1 more source
Animal Models and Experimental Medicine, EarlyView.This study developed a minimally invasive, in situ small intestinal injection technique for mice, enabling targeted delivery of bioactive molecules while bypassing gastric degradation. Validation using functional assays and single‐cell RNA sequencing reveals high‐mobility group box 1–mediated epithelial responses, offering a translational tool for gut ...
Yawen Lai +7 more
wiley +1 more source