A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems
MethodsXProteic plasmid addiction systems, such as the control of cell death (Ccd), have been used for efficient plasmid DNA recombination. The CcdB toxin, which has a relatively long sequence of 309 bp, has been the predominant choice for this purpose. However,
Agnieszka M. Murakami +4 more
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Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning. [PDF]
, 1995 Identifying the immunogenic proteins that elicit pathogen-specific T cell responses is key to rational vaccine design. While several approaches have succeeded in identifying major histocompatibility complex (MHC) class I bound peptides that stimulate CD8+
Campbell, D +2 more
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The complete structure of an activated open sodium channel
Nature Communications, 2017 Voltage-gated sodium (Nav) channels are crucial for action potential initiation in excitable cells. Here the authors present the complete structure of prokaryotic NavMs in a fully open state, providing structural insight into the opening and closure of ...
Altin Sula +5 more
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Aerobically respiring prokaryotic strains exhibit a broader temperature–pH–salinity space for cell division than anaerobically respiring and fermentative strains [PDF]
, 2015 Jesse P. Harrison +6 more
openalex +1 more source
Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody
Animal Research and One Health, EarlyView.This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng +7 more
wiley +1 more source
Toxins VapC and PasB from Prokaryotic TA Modules Remain Active in Mammalian Cancer Cells [PDF]
, 2014 Łukasz Wieteska +3 more
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Biotechnology and Applied Biochemistry, EarlyView.Abstract Oil spills pose severe threats to marine ecosystems and coastal communities. Alcanivorax borkumensis SK2, a marine bacterium with superior hydrocarbon‐degrading capabilities, emerges as a promising agent for bioremediation. This study identified an economical growth substrate for A.
Élisabeth Perreault +2 more
wiley +1 more source
RRS Discovery Cruise 368, 15 Jul - 04 Aug 2011. Hydrographic measurements on WOCE line A16N
, 2012 RRS Discovery Cruise 368 was a repeat occupation of part of the Atlantic hydrographic section designated by the World Ocean Circulation Experiment (WOCE) as A16N. A total of 29 CTDO (conductivity-temperature-depth-oxygen) stations were occupied.
et al,, King, B.A.
core
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas
, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
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Proliferation without nuclei suggests mitochondrial cells (MitoCells) to be prokaryotic nature [PDF]
, 2017 Keiko Nakano +8 more
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