Guidelines for Light‐Start One‐Pot RPA‐CRISPR‐Cas12a Assays for Foodborne Pathogen Detection
Food Safety and Health, EarlyView.Light‐start one‐pot RPA‐CRISPR‐Cas12a detection schematic diagram. (A) Linear crRNA activation light‐start one‐pot workflow. (B) Caged crRNA activation light‐start one‐pot workflow. ABSTRACT Rapid and accurate detection of nucleic acids of foodborne pathogens is of vital importance for controlling the spread of foodborne diseases and safeguarding ...
Fan Li +4 more
wiley +1 more source
Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields
Cell Communication and SignalingEvery single cell can communicate with other cells in a paracrine manner via the production of nano-sized extracellular vesicles. This phenomenon is conserved between prokaryotic and eukaryotic cells.
Halimeh Mobarak +10 more
doaj +1 more source
Metagenomic mining reveals novel Cas12 subtypes and their evolutionary diversification
iMetaOmics, EarlyView.We identified 4112 Cas12 Proteins and 6 new Cas12 subtypes, revealed their significant diversity in N‐terminal regions, repeat sequences, and sequences motifs. We developed an AI‐driven algorithm, Cas12fam, which allows precise annotation of 18 distinct domains in Cas12 proteins.
Meixia Yu +11 more
wiley +1 more source
Convergent evolution of metabolic functions: Evidence from the gut microbiomes of humans and dogs
iMetaOmics, EarlyView.Dogs have undergone convergent evolution in their genome with humans in long‐term co‐living. Studies have shown that the gut microbiomes of dogs exhibit greater similarity to those of humans compared to mice and pigs. In our study, we first compared the gut microbiomes of macaques, three dog groups, and two wolf groups to those in humans.
Xiaoyang Wang +10 more
wiley +1 more source
Cell‐Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems [PDF]
, 2015 Anne Zemella +3 more
openalex +1 more source
Single-molecule live-cell imaging visualizes parallel pathways of prokaryotic nucleotide excision repair [PDF]
, 2020 Harshad Ghodke +2 more
openalex +1 more source
iMetaOmics, EarlyView.Estuaries are vital hotspots for antibiotic resistance genes (ARGs) due to substantial antibiotic pollution. Although viruses have been proposed as key reservoirs and important disseminators of ARGs in environments, their contribution to the estuarine antibiotic resistome remains largely unknown.
Xiao‑Qing Luo +9 more
wiley +1 more source
Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells. [PDF]
Synth Syst Biotechnol, 2019 Miao C, Zhao H, Qian L, Lou C.
europepmc +1 more source
Chromosome Architecture and Segregation in Prokaryotic Cells
Journal of Molecular Microbiology and Biotechnology, 2015 P. Graumann
semanticscholar +1 more source
Epigenetic Regulation in the Pathogenesis of Renal Inflammation: Insights and Therapeutic Potentials
iNew Medicine, EarlyView.ABSTRACT Renal inflammation is a common pathological process in various kidney diseases, often initiated by factors such as toxins, ischemia, or autoimmune reactions. This inflammatory response can result in structural damage and a rapid decline in renal function.
Yu‐Hang Dong +5 more
wiley +1 more source