Results 241 to 250 of about 10,473,632 (314)

Navigating Past Oceans: Comparing Metabarcoding and Metagenomics of Marine Ancient Sediment Environmental DNA

Molecular Ecology Resources, EarlyView.
ABSTRACT The condition of ancient marine ecosystems provides context for contemporary biodiversity changes in human‐impacted oceans. Sequencing sedimentary ancient DNA (sedaDNA) is an emerging method for generating high‐resolution biodiversity time‐series data, offering insights into past ecosystems.
Luke E. Holman, Giulia Zampirolo, Richard Gyllencreutz, James Scourse, Tobias Frøslev, Christian Carøe, Shyam Gopalakrishnan, Mikkel Winther Pedersen, Kristine Bohmann   +8 more
wiley   +1 more source

Chimeric origins and dynamic evolution of central carbon metabolism in eukaryotes. [PDF]

Nat Ecol Evol
Santana-Molina C, Williams TA, Snel B, Spang A.   +3 more
europepmc   +1 more source

Evaluating the Benefits and Limits of Multiple Displacement Amplification With Whole‐Genome Oxford Nanopore Sequencing

Molecular Ecology Resources, EarlyView.
ABSTRACT Multiple displacement amplification (MDA) outperforms conventional PCR in long fragment and whole‐genome amplification, making it attractive to couple MDA with long‐read sequencing of samples with limited quantities of DNA to obtain improved genome assemblies.
Fiifi Agyabeng‐Dadzie, Megan S. Beaudry, Alex Deyanov, Haley Slanis, Minh Q. Duong, Randi Turner, Asis Khan, Cesar A. Arias, Jessica C. Kissinger, Travis C. Glenn, Rodrigo de Paula Baptista   +10 more
wiley   +1 more source

Separate Kingdoms, Same Conspiracies: Conserved Viral Strategies for Immune Evasion in Animal and Bacterial Hosts. [PDF]

MedComm (2020)
Wang J, He X, Li G.
europepmc   +1 more source

A Practical Comparison of Short‐ and Long‐Read Metabarcoding Sequencing: Challenges and Solutions for Plastid Read Removal and Microbial Community Exploration of Seaweed Samples

Molecular Ecology Resources, EarlyView.
ABSTRACT Short‐read metabarcoding analysis is the gold standard for accessing partial 16S and ITS genes with high read quality. With the advent of long‐read sequencing, the amplification of full‐length target genes is possible, but with low read accuracy.
Coralie Rousseau, Nicolas Henry, Sylvie Rousvoal, Gwenn Tanguy, Erwan Legeay, Catherine Leblanc, Simon M. Dittami   +6 more
wiley   +1 more source

Secreted Expression of Thymosin β4 from <i>Pinctada fucata</i> in <i>Pichia pastoris</i> and Its Biological Activity. [PDF]

Biology (Basel)
Liu P, Mo X, Liu J, Li W, Tang J, Li Q, Lin J.   +6 more
europepmc   +1 more source

Quantifying Soil Microbiome Abundance by Metatranscriptomics and Complementary Molecular Techniques—Cross‐Validation and Perspectives

Molecular Ecology Resources, EarlyView.
ABSTRACT Linking meta‐omics and biogeochemistry approaches in soils has remained challenging. This study evaluates the use of an internal RNA extraction standard and its potential for making quantitative estimates of a given microbial community size (biomass) in soil metatranscriptomics. We evaluate commonly used laboratory protocols for RNA processing,
Mathilde Borg Dahl, Stella Brachmann, Andrea Söllinger, Marina Schnell, Laureen Ahlers, Magdalena Wutkowska, Katharina J. Hoff, Neetika Nath, Verena Groß, Haitao Wang, Micha Weil, Marc Piecha, Marc Schaffer, Corinna Jensen, Andreas W. Kuss, Christoph Gall, Erika Wimmer, Thomas Pribasnig, Alexander Tøsdal Tveit, Bjarni D. Sigurdsson, Christa Schleper, Andreas Richter, Tim Urich   +22 more
wiley   +1 more source

Impact of Farming System on Soil Microbial Communities Associated with Common Bean in a Region of Northern Spain. [PDF]

Plants (Basel)
Suarez-Fernandez M, Ferreira JJ, Campa A.   +2 more
europepmc   +1 more source

Metformin and Dietary Restriction Counteract Aging via Reducing m6A–Dependent Stabilization of Methionine Synthase mRNA in Brachionus asplanchnoidis (Rotifera)

Aging Cell, EarlyView.
Metformin and dietary restriction modulate m6A to extend lifespan of rotifer, highlighting MTR as a central regulator of aging through methionine metabolism. ABSTRACT Metformin, a medication primarily used to treat diabetes, has gained attentions for its potential antiaging properties.
Yu Zhang, Xiaojie Liu, Hairong Lian, Yanchao Chai, Yang Zhou, Dongqi Kan, Jilong Ren, Cui Han, Jiaxin Yang   +8 more
wiley   +1 more source

BLOC1S1 Control of Vacuolar Organelle Fidelity Modulates Murine TH2 Cell Immunity and Allergy Susceptibility

Allergy, EarlyView.
Abbreviations: BLOC1S1, biogenesis of lysosome organelles complex 1 subunit 1; cGAS, cyclic GMP‐AMP synthase; GATA3, GATA binding protein 3; IgE, immunoglobulin E; IKK, inhibitor of nuclear factor kappa‐B kinase; IL, interleukin; mtDNA, mitochondrial DNA; NF‐κB, nuclear factor‐kappa B; OVA, ovalbumin; MC903, vitamin D3 analog; STING, stimulator of ...
Rahul Sharma, Kaiyuan Wu, Kim Han, Anna Chiara Russo, Pradeep K. Dagur, Christian A. Combs, Xianglan Yao, Stewart J. Levine, Michael N. Sack   +8 more
wiley   +1 more source