Results 41 to 50 of about 10,473,632 (314)
Understanding microbial community dynamics in the alpine cryosphere is an important step toward assessing climate change impacts on these fragile ecosystems and meltwater-fed environments downstream.
Lucas Fillinger+8 more
doaj +1 more source
A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments. [PDF]
Sea ice is an analog environment for several of astrobiology's near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons.
Christian A Lindensmith+7 more
doaj +1 more source
Prokaryotic Argonaute Proteins as a Tool for Biotechnology
Programmable nucleases are the most important tool for manipulating the genes and genomes of both prokaryotes and eukaryotes. Since the end of the 20th century, many approaches were developed for specific modification of the genome.
E. Kropocheva+5 more
semanticscholar +1 more source
A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host Cells In Vitro
Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells.
James W. Marsh+2 more
doaj +1 more source
Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. [PDF]
Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the ...
Henriette O'Geen+4 more
doaj +1 more source
Trapping and isolation of single prokaryotic cells in a micro-chamber array using dielectrophoresis
The vast majority of prokaryotic species are difficult or impossible to culture in laboratories, which makes it difficult to study these organisms using conventional biochemical techniques. Methods that enable the physical isolation of single prokaryotic
K. Mogi+5 more
semanticscholar +1 more source
Exploring the Prokaryotic Community Associated With the Rumen Ciliate Protozoa Population
Ciliate protozoa are an integral part of the rumen microbiome and were found to exert a large effect on the rumen ecosystem itself as well as their host animal physiology.
Bar Levy, Bar Levy, Elie Jami
doaj +1 more source
The MADS-box transcription factor family plays an important role in regulating growth and signal transduction in animals and plants. TaMADS32 encodes a type II MADS-box transcription factor which is mainly expressed in wheat root system.
Xuemei Zhang+8 more
doaj +1 more source
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui+5 more
wiley +1 more source