Results 51 to 60 of about 10,824,096 (352)

The RCK2 domain of the human BKCa channel is a calcium sensor [PDF]

, 2008
Large conductance voltage and Ca2+-dependent K+ channels (BKCa) are activated by both membrane depolarization and intracellular Ca2+. Recent studies on bacterial channels have proposed that a Ca2+-induced conformational change within specialized ...
Albright, Braun, C. S. Gandhi, Chakrapani, Corbett, Diaz, Gales, Gribkoff, Jiang, Jiang, Kelly, Latorre, M. Ottolia, N. Savalli, Pallotta, Patton, R. Olcese, Roosild, Sali, Salkoff, Schreiber, Schreiber, Shen, Shi, Sreerama, Sreerama, Stefani, T. Yusifov, Tang, Tomaselli, Venyaminov, Wallner, Xia, Ye, Zimmer   +34 more
core   +3 more sources

Trapping and isolation of single prokaryotic cells in a micro-chamber array using dielectrophoresis

, 2016
The vast majority of prokaryotic species are difficult or impossible to culture in laboratories, which makes it difficult to study these organisms using conventional biochemical techniques. Methods that enable the physical isolation of single prokaryotic
K. Mogi, C. Shirataki, Kumiko Kihara, H. Kuwahara, Yuichi Hongoh, Takatoki Yamamoto   +5 more
semanticscholar   +1 more source

A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host Cells In Vitro

Frontiers in Microbiology, 2017
Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells.
James W. Marsh, Michael S. Humphrys, Garry S. A. Myers   +2 more
doaj   +1 more source

Editorial: Proteases of the prokaryotic cell envelope

Frontiers in Microbiology, 2022
مقالة افتتاحية Front. Microbiol., 02 August 2022Sec.
Rosana E. De Castro, Ansgar Poetsch, Ansgar Poetsch, Ansgar Poetsch   +3 more
openaire   +3 more sources

Strong negative self regulation of Prokaryotic transcription factors increases the intrinsic noise of protein expression [PDF]

, 2008
Background Many prokaryotic transcription factors repress their own transcription. It is often asserted that such regulation enables a cell to homeostatically maintain protein abundance.
A Bar-Even, A Becskei, A de la Hoz, A Kierzek, C Cox, D Austin, Dafyd J Jenkins, Dov J Stekel, E Ozdubak, F Neidhardt, G Shinar, H El-Samad, I Keseler, J Paulsson, J Plumbridge, K Kostelidou, L Bingle, L Cai, M Elowitz, M Gibson, M Keeling, M Koern, M Samoilov, M Simpson, M Simpson, M Thattai, M Wall, N Rosenfeld, P Chivers, P Swain, Q Wang, R Rolfes, S Hooshangi, S Reichheld, T Kepler, Y Dublanche   +35 more
core   +4 more sources

A Stochastic model for dynamics of FtsZ filaments and the formation of Z-ring

, 2020
Understanding the mechanisms responsible for the formation and growth of FtsZ polymers and their subsequent formation of the $Z$-ring is important for gaining insight into the cell division in prokaryotic cells.
Kumar, A. V. Anil, Sumedha, Swain, Arabind   +2 more
core   +1 more source

Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. [PDF]

PLoS ONE, 2018
Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the ...
Henriette O'Geen, Chonghua Ren, Nicole B Coggins, Sofie L Bates, David J Segal   +4 more
doaj   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman, Atkinson, Baekkeskov, Baekkeskov, Berthold, Boitard, Bottazzo, Castano, Dean, Frorath, Goedel, Greenbaum, Harrison, Heike Berg, Hopp, Jochen Seissler, Karjalainen, Kaufman, Kuglin, Kuglin, Ludwig Mauch, Michael Walter, Palmer, Patrick, Seissler, Smith, Smith, Snyder, Su, Wolfgang Northemann   +29 more
core   +1 more source

Exploring the Prokaryotic Community Associated With the Rumen Ciliate Protozoa Population

Frontiers in Microbiology, 2018
Ciliate protozoa are an integral part of the rumen microbiome and were found to exert a large effect on the rumen ecosystem itself as well as their host animal physiology.
Bar Levy, Bar Levy, Elie Jami
doaj   +1 more source

Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity [PDF]

, 2007
BACKGROUND The Cation Diffusion Facilitator (CDF) family is a ubiquitous family of heavy metal transporters. Much interest in this family has focused on implications for human health and bioremediation.
Blaudez, D., Chalot, M., Jeandroz, S., Montanini, B., Sanders, D.   +4 more
core   +3 more sources