Results 211 to 220 of about 795,608 (343)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Epigenetic evolution and clinicopathological implications of distinct DNA methylation profiles in pancreatic ductal adenocarcinoma. [PDF]

Sci Rep
Kitahama K, Ho YJ, Satomi K, Shibayama T, Nagahama K, Ohtsuka K, Ohnishi H, Sakamoto Y, Shibahara J, Hayashi A.   +9 more
europepmc   +1 more source

Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase

FEBS Open Bio, EarlyView.
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni, Florian Csarman, Peicheng Sun, Mirjam Kabel, Roland Ludwig   +4 more
wiley   +1 more source

CanASM: a comprehensive database for genome-wide allele-specific DNA methylation identification and annotation in cancer. [PDF]

BMC Genomics
Zhao J, Zhao Z, Li H, Yu H, Lin H, Chen H, Li X, Liu D, Wang Y, Wang G.   +9 more
europepmc   +1 more source

Matrix: a complex amalgam of structures and functions in tumor microenvironment

FEBS Open Bio, EarlyView.
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis, Evgenia Tsoukala, Theodora D. Sarantopoulou, Maria‐Elpida Christopoulou   +3 more
wiley   +1 more source

Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Karacabey Merino. [PDF]

BMC Vet Res
Yaman Y, Aymaz R, Keleş M, Kişi YE, Hatipoğlu E, Özdemir A, Çetinkaya E.   +6 more
europepmc   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

Viral Enhancers Orchestrate Kaposi's Sarcoma-Associated Herpesvirus Transcription. [PDF]

J Med Virol
Chowdhury NR, Shamay M.
europepmc   +1 more source

Liver‐specific lncRNAs associated with liver cancers

FEBS Open Bio, EarlyView.
Long non‐coding RNAs (lncRNAs) are regulatory molecules with various functions. They are more tissue‐specific than proteins and can be used as potential biomarkers, particularly in cancer diagnostics and prognosis. In this review, we have systematically compiled all lncRNAs with exclusive expression in the human liver, verified their liver specificity ...
Olga Y. Burenina, Roman K. Makarchenko, Maria P. Rubtsova, Olga A. Dontsova   +3 more
wiley   +1 more source

Characterization of the MgsR-dependent promoter structure in Bacillus subtilis-application of a novel pHIS plasmid-based screening system for promoter element analysis. [PDF]

Nucleic Acids Res
Harms M, Wolfgramm H, Schedlowski M, Michalik S, Hildebrandt P, Schaffer M, Völker U, Reder A.   +7 more
europepmc   +1 more source