Results 181 to 190 of about 4,678 (214)

Effects of flurbiprofen and flurbinitroxybutylester on prostaglandin endoperoxide synthases

European Journal of Pharmacology, 1996
The aim of our study was to evaluate the selectivity of flurbiprofen and flurbinitroxybutylester for inhibition of the cyclooxygenase activity of prostaglandin endoperoxide synthase-2 vs. prostaglandin endoperoxide synthase-1 in human blood monocytes and platelets, respectively.
Santini, G, Sciulli, Mg, Panara, Mr, Padovano, R, di Giamberardino, M, Rotondo, Mt, Del Soldato, P, PATRIGNANI, Paola   +7 more
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The enzymology of prostaglandin endoperoxide H synthases-1 and -2

Prostaglandins & Other Lipid Mediators, 2002
We summarize the enzymological properties of prostaglandin endoperoxide H synthases (PGHs)-1 and -2, the enzymes that catalyze the committed step in prostaglandin biosynthesis. These isoenzymes are closely related structurally and mechanistically.
William L, Smith, Inseok, Song
openaire   +2 more sources

Activation of 2-Aminofluorene by Prostaglandin Endoperoxide H Synthase 2

Biochemical and Biophysical Research Communications, 1995
Prostaglandin endoperoxide H synthase is the key enzyme in the conversion of arachidonic acid to tissue prostanoids. Two isoforms of prostaglandin endoperoxide H synthase have been identified: PHS-1 is constitutively expressed in most tissues under normal physiological conditions and PHS-2 is expressed in response to inflammatory agents, tumor ...
Y, Liu, G N, Levy, W W, Weber
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Immunocytochemical localization of prostaglandin endoperoxide synthase in the bovine intestine

Histochemistry, 1993
The localization of prostaglandin (PG) endoperoxide synthase in bovine intestine was examined immunocytochemically with polyclonal antibody raised against PG endoperoxide synthase purified from bovine seminal glands. The most intense positive staining reaction for the enzyme was present in mast cells.
K, Ishimura, T, Suzuki, K, Fukui, A, Yamamoto, Y, Omoto, N, Ueda, S, Yamamoto   +6 more
openaire   +2 more sources

Prostaglandin endoperoxide H synthase expression in human thyroid epithelial cells

American Journal of Physiology-Cell Physiology, 2001
KAT-50, an established human thyrocyte cell line, expresses constitutively high levels of prostaglandin endoperoxide H synthase-2 (PGHS-2), the inflammatory cyclooxygenase. Here, we examine primary human thyrocytes. We find that they, too, express PGHS-2 mRNA and protein under control culture conditions.
A G, Gianoukakis, H J, Cao, T A, Jennings, T J, Smith   +3 more
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Not prostacyclin synthase but prostaglandin endoperoxide synthase increases with human placental development

Prostaglandins, 1986
Prostaglandin endoperoxide synthase (i.e. cyclooxygenase; PGH synthase) and prostacyclin synthase (PGI synthase) were quantitated with specific immunoradiometric assays in microsomes from human placentae (n = 20) obtained from 7 up to 17 weeks of gestation.
M J, Keirse, J J, Erwich, G, Klok
openaire   +2 more sources

Characterization of a Tyrosyl Radical in Prostaglandin Endoperoxide Synthase-2

Biochemical and Biophysical Research Communications, 1994
Two different isoforms of prostaglandin H synthase, prostaglandin H synthase-1 and prostaglandin H synthase-2, have been identified. Both isozymes catalyze both cyclooxygenase and peroxidase reactions. Residues identified as being essential for catalysis by ovine prostaglandin endoperoxide H synthase-1 are all conserved in prostaglandin H synthase-2 ...
L C, Hsi, C W, Hoganson, G T, Babcock, W L, Smith   +3 more
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Inhibition of lipoxygenase and prostaglandin endoperoxide synthase by anacardic acids

Biochemical and Biophysical Research Communications, 1991
C22:1 omega 5-anacardic acid was found to be a good inhibitor of both potato lipoxygenase and ovine prostaglandin endoperoxide synthase with approximate IC50's of 6 and 27 microM, respectively. Very similar inhibition was seen with the crude exudate, rich in omega 5-anacardic acids, from glandular trichomes of an arthropod-resistant strain of geranium,
R, Grazzini, D, Hesk, E, Heininger, G, Hildenbrandt, C C, Reddy, D, Cox-Foster, J, Medford, R, Craig, R O, Mumma   +8 more
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Rapid Inactivation of Prostaglandin Endoperoxide Synthases by N-(Carboxyalkyl)maleimides

Biochemistry, 1994
N-(Carboxyalkyl)maleimides were synthesized as potential inhibitors of prostaglandin endoperoxide synthase (PGHS). Inactivation of the cyclooxygenase and peroxidase activities of PGHS occurred in a biphasic manner with extremely rapid inactivation followed by slow, time-dependent inactivation.
A S, Kalgutkar, L J, Marnett
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Expression of prostaglandin endoperoxide synthase-1 in a baculovirus system

Biochemical and Biophysical Research Communications, 1992
A cDNA coding for ovine prostaglandin endoperoxide (PGH) synthase-1 was used to construct a recombinant baculovirus which was expressed in Spodoptera frugiperda (Sf9) insect cells. Two proteins reactive with anti-PGH synthase antibody were produced. A larger protein (Mr = 72,000) coelectrophoresed with native enzyme; a smaller, more abundant protein ...
T, Shimokawa, W L, Smith
openaire   +2 more sources