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Hsp70 proteins in protein translocation

2001
Publisher Summary Hsp70 proteins are essential components of the protein translocation systems of the endoplasmic reticulum (ER) and mitochondria. The role of Hsp70s in protein translocation is not confined to the ER and mitochondria. For most proteins of the ER, translocation is coupled with protein synthesis.
Pfanner, Nikolaus.   +1 more
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Protein Arrays: Labeling the Protein and Probing the Array for Protein-Protein Interactions

Cold Spring Harbor Protocols, 2007
INTRODUCTIONTo detect protein-protein interactions, a protein microarray can be incubated with a fluorescently labeled protein. After the array is washed, stable interactions can be identified by scanning the slide for fluorescent spots. This protocol uses cyanine-3 (Cy3) or cyanine-5 (Cy5) monofunctionally activated dyes to label the probe proteins.
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Protein–Protein Interactions: Protein interactions contribute to protein function

Trends in Genetics, 2002
Protein–Protein Interactions (A Molecular Cloning Manual) edited by Erica GolemisCold Spring Harbor Laboratory Press, 2001. £100.00 pbk (600 pages)ISBN 0 879 69628 1Proteins control and mediate many of the biological activities of cells. Hence, to gain an understanding of cellular function, the function of every protein must be understood, both in ...
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Protein

2003
Proteins are the ultimate players in the processes that allow an organism to function and reproduce. Their use in medical therapy requires their isolation in pure form. This chapter addresses the calculations needed to quantify protein and, for the cases of several examples, to assess protein activity.
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