Results 191 to 200 of about 2,852,076 (267)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A comprehensive view on r-protein binding and rRNA domain structuring during early eukaryotic ribosome formation. [PDF]

Nucleic Acids Res
Gerhalter M, Prattes M, Grundmann LE, Grishkovskaya I, Semeraro EF, Zisser G, Kotisch H, Merl-Pham J, Hauck SM, Haselbach D, Bergler H.   +10 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Photoactivatable, Biomimetic Ligand photoMultiTASQ Traps DNA/RNA G‑Quadruplexes and Their Protein Binding Partners. [PDF]

ACS Bio Med Chem Au
Raevens S, Danino YM, Desingle C, Rota Sperti F, Pirrotta M, Mazzucotelli L, Letissier L, Hornstein E, Cianferani S, Hernandez-Alba O, Valverde IE, Monchaud D.   +11 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Hydroxyl and Trifluoromethyl Radical Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides. [PDF]

Anal Chem
Yusuph Q, Misra SK, Liu H, Sharp JS.
europepmc   +1 more source

MPBind: a multitask protein binding site predictor using protein language models and equivariant GNNs. [PDF]

Bioinformatics
Wang Y, Boadu F, Cheng J.
europepmc   +1 more source

Cancer histone mutations impact protein binding and DNA repair with possible links to genomic instability. [PDF]

Nucleic Acids Res
Espiritu D, Sheng Y, Peng Y, Ostroverkhova D, Li S, Landsman D, Aristizabal MJ, Panchenko AR.   +7 more
europepmc   +1 more source

Kurarinone and sophoraflavanone G from <i>Sophora flavescens</i> suppress <i>Streptococcus agalactiae</i> biofilm via dual antibiofilm mechanisms involving adhesion protein binding and ABC transporter pathway perturbation. [PDF]

Front Microbiol
Shi J, Zhang Y, An J, Li L, Li A.
europepmc   +1 more source

Adaptation of DNA to Protein Binding Revealed by Spectroscopy and Molecular Simulation. [PDF]

J Phys Chem B
van Heesch T, Sharma S, van Erp B, Ortíz APA, Dame RT, Vreede J, Gavvala K.   +6 more
europepmc   +1 more source