Results 81 to 90 of about 754,932 (265)

Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast

Molecular Systems Biology, 2011
To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with dynamic kinetic modeling.
Christian Miller, Björn Schwalb, Kerstin Maier, Daniel Schulz, Sebastian Dümcke, Benedikt Zacher, Andreas Mayer, Jasmin Sydow, Lisa Marcinowski, Lars Dölken, Dietmar E Martin, Achim Tresch, Patrick Cramer   +12 more
doaj   +1 more source

A Comprehensive Review of BET Protein Biochemistry, Physiology, and Pathological Roles. [PDF]

Front Pharmacol, 2022
Ali HA, Li Y, Bilal AHM, Qin T, Yuan Z, Zhao W.   +5 more
europepmc   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

uPIC-M: Efficient and Scalable Preparation of Clonal Single Mutant Libraries for High-Throughput Protein Biochemistry. [PDF]

ACS Omega, 2021
Appel MJ, Longwell SA, Morri M, Neff N, Herschlag D, Fordyce PM.   +5 more
europepmc   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Vaccines efficacy to SARS-CoV-2 variants require holistic knowledge of viral immunology and protein biochemistry. [PDF]

Hum Vaccin Immunother, 2021
Kumar S.
europepmc   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Protein Biochemistry and Molecular Modeling of the Intra-Melanosomal Domain of Human Recombinant Tyrp2 Protein and OCA8-Related Mutant Variants. [PDF]

Int J Mol Sci, 2022
Dolinska MB, Woods T, Osuna I, Sergeev YV.   +3 more
europepmc   +1 more source

Biochemistry of the chromogranin A protein family [PDF]

Biochemical Journal, 1989
J P, Simon, D, Aunis
openaire   +2 more sources

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source