Results 61 to 70 of about 790,336 (266)

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Protocol for unified metabolomics and proteomics analysis of formalin-fixed paraffin-embedded tissue

STAR Protocols
Summary: The use of archival formalin-fixed paraffin-embedded (FFPE) tissue samples for biochemical analyses is problematic because of the formation of a Schiff base, leading to low protein and metabolite yields during analytical extractions.
Abisola R. Isaiah, Laneke Luies, Du Toit Loots, Aurelia A. Williams, Maré Vlok, Novel N. Chegou, Marceline Tutu van Furth, Martijn van der Kuip, Shayne Mason   +8 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Proteome-wide analysis of Anopheles culicifacies mosquito midgut: new insights into the mechanism of refractoriness

BMC Genomics, 2018
Background Midgut invasion, a major bottleneck for malaria parasites transmission is considered as a potential target for vector-parasite interaction studies.
Sonam Vijay, Ritu Rawal, Kavita Kadian, Jagbir Singh, Tridibesh Adak, Arun Sharma   +5 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Insights into the structural differences between homo- and heterodimers enriched from a cocktail of monoclonal antibodies against SARS-CoV-2

Scientific Reports
Heteromeric complexes observed within co-formulated products represent a unique class of impurities that are poorly understood in terms of their biochemical, biophysical, structural, and functional attributes.
Jennifer B. Nguyen, Sophia Liu, Yuetian Yan, Deepanshu Choudhary, Zachary R. Oberholtzer, Yi Zhou, Donna J. Skow, Mushhood Sheikh, Cristinel Sandu, Shunhai Wang, Ning Li, Matthew C. Franklin, Michael P. Rosconi, Erica A. Pyles   +13 more
doaj   +1 more source

Vectorized Delivery of Alpha-GalactosylCeramide and Tumor Antigen on Filamentous Bacteriophage fd Induces Protective Immunity by Enhancing Tumor-Specific T Cell Response

Frontiers in Immunology, 2018
We have exploited the properties of filamentous bacteriophage fd to deliver immunologically active lipids together with antigenic peptides. Filamentous bacteriophages resemble for size, capability to be permeable to blood vessels, and high density ...
Rossella Sartorius, Luciana D’Apice, Pasquale Barba, Deborah Cipria, Laura Grauso, Adele Cutignano, Piergiuseppe De Berardinis   +6 more
doaj   +1 more source

Editorial: Insights in protein biochemistry: protein biophysics 2022. [PDF]

Front Mol Biosci, 2023
Labrou NE, Kwok HF, Zhang Q.
europepmc   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Alan Fincham and the era of enamel protein Biochemistry. [PDF]

Front Physiol, 2022
Diekwisch TGH.
europepmc   +1 more source