Results 161 to 170 of about 16,988,925 (342)

Nonergodic complex medium dynamics affecting protein electron transfer [PDF]

The standard description of long-range protein electron transfer anticipates an exponential decay of the reaction rate with the donor-acceptor distance due to decaying tunneling probability.
Dmitry, Matyushov
core   +1 more source

Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method

Nature Communications
Transfer RNA (tRNA) plays a central role in translation. The simultaneous in vitro synthesis of minimal yet sufficient tRNA species (at least 21) poses a challenge for constructing a self-reproducible artificial cell.
Ryota Miyachi, Keiko Masuda, Yoshihiro Shimizu, Norikazu Ichihashi   +3 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

A cyclic peptide-grafted Fc with hepatocyte growth factor functionality ameliorates hepatic fibrosis in a non-alcoholic steatohepatitis mouse model

iScience
Summary: The regenerative functions associated with cytokines and growth factors have immense therapeutic potential; however, their poor pharmacokinetics, resulting from structural features, hinder their effectiveness.
Nichole Marcela Rojas-Chaverra, Ryu Imamura, Hiroki Sato, Toby Passioura, Emiko Mihara, Tatsunori Nishimura, Junichi Takagi, Hiroaki Suga, Kunio Matsumoto, Katsuya Sakai   +9 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Interactive visualization of protein dynamics [PDF]

Proceedings Visualization 2000. VIS 2000 (Cat. No.00CH37145), 2002
R. van Liere (Robert), H. Huitema (Henk)
openaire   +2 more sources

Prospecting the protein design landscape

FEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona, Katharina T. Stonig, Jens Meiler, Clara T. Schoeder, Monica L. Fernández‐Quintero   +4 more
wiley   +1 more source

Simulating the Peptide Folding Kinetic Related Spectra Based on the Markov State Model

, 2014
Optical spectroscopic tools are used to monitor protein folding/unfolding dynamics after a fast triggering such as the laser induced temperature jump. These techniques provide new opportunities for comparison between theory and simulations and atom-level
Han, KL, Zhuang, Wei, Yang, MJ, Zhang, X, Song, Jian   +4 more
core   +1 more source

Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements

Scientific Reports
To develop artificial cell models that mimic living cells, cell-sized lipid vesicles encapsulating cell-free protein synthesis (CFPS) systems are useful for protein expressions or artificial gene circuits for vesicle–vesicle communications.
Akari Miwa, Masatoshi Wakamori, Tetsuro Ariyoshi, Yasushi Okada, Mikako Shirouzu, Takashi Umehara, Koki Kamiya   +6 more
doaj   +1 more source