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Engineering therapeutic proteins
Current Opinion in Structural Biology, 2000Many early drug candidates derived from biotechnology failed in clinical trials because of their low affinity/specificity, short half-lives or immunogenicity. Protein engineering techniques have been applied to circumvent some of the problems that hindered these earlier trials, resulting in clinical benefits from a range of engineered antibodies and ...
J, McCafferty, D R, Glover
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Trends in Biotechnology, 1997
Membrane proteins perform many of the essential functions required for life. They are often the targets of medicinal drugs and have many potential uses in biotechnological processes. Therefore our ability to understand them and manipulate their functions is both important and necessary to enable protein engineers to create 'designer' membrane proteins (
I, Mingarro, G, von Heijne, P, Whitley
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Membrane proteins perform many of the essential functions required for life. They are often the targets of medicinal drugs and have many potential uses in biotechnological processes. Therefore our ability to understand them and manipulate their functions is both important and necessary to enable protein engineers to create 'designer' membrane proteins (
I, Mingarro, G, von Heijne, P, Whitley
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Science, 1983
The prospects for protein engineering, including the roles of x-ray crystallography, chemical synthesis of DNA, and computer modeling of protein structure and folding, are discussed. It is now possible to attempt to modify many different properties of proteins by combining information on crystal structure and protein chemistry with artificial gene ...
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The prospects for protein engineering, including the roles of x-ray crystallography, chemical synthesis of DNA, and computer modeling of protein structure and folding, are discussed. It is now possible to attempt to modify many different properties of proteins by combining information on crystal structure and protein chemistry with artificial gene ...
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Current Opinion in Biotechnology, 1995
Much of the research on integral membrane proteins mirrors that on soluble proteins; however, membrane protein engineering also has its own ends and means, many of which take advantage of the peculiar situation of membrane proteins, whose chains are distributed between one lipidic and two aqueous phases. Extramembrane loops have been shortened, cut, or
J L, Popot, M, Saraste
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Much of the research on integral membrane proteins mirrors that on soluble proteins; however, membrane protein engineering also has its own ends and means, many of which take advantage of the peculiar situation of membrane proteins, whose chains are distributed between one lipidic and two aqueous phases. Extramembrane loops have been shortened, cut, or
J L, Popot, M, Saraste
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Biotechnology Advances, 1987
The techniques of protein engineering are proving to be a revolutionary experimental tool for understanding protein structure-function relationships. Even at this early stage, proteins of improved characteristics for specific industrial and therapeutic uses have already been produced.
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The techniques of protein engineering are proving to be a revolutionary experimental tool for understanding protein structure-function relationships. Even at this early stage, proteins of improved characteristics for specific industrial and therapeutic uses have already been produced.
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Protein engineering of ribonucleases
Biochimie, 1998Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase.
CAFARO, VALERIA +5 more
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Engineering Fluorescent Proteins
2005Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores intrinsically within their respective protein sequences. Recent studies have made progress in obtaining bright variants of these proteins which develop chromophores quickly and efficiently, as well as novel
Atsushi, Miyawaki +2 more
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Engineering BRET-Sensor Proteins
2017FRET-sensors have become important tools for intracellular imaging, but their dependence on external illumination presents some limitations, such as photobleaching and phototoxicity, which limit measurements over extended periods of time. Fluorescence measurements also suffer from autofluorescence and light scattering, which hampers in vivo imaging and
Remco Arts +2 more
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2010
This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004-2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation studies and from study of thermophilic (and other extremophilic) proteins.
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This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004-2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation studies and from study of thermophilic (and other extremophilic) proteins.
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"Protein Engineering, Design and Selection", 1986
R J, Leatherbarrow, A R, Fersht
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R J, Leatherbarrow, A R, Fersht
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