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Protein expression in plastids

Current Opinion in Plant Biology, 2001
The genome of the plastid has generated much interest as a target for plant transformation. The characteristics of plastid transgenes both reflect the prokaryotic origin of plastid organelles and provide a unique set of features that are currently lacking in genes introduced into the plant nucleus. Recent progress has been made in understanding plastid
P B, Heifetz, A M, Tuttle
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Protein Expression-Yeast

2014
Yeast is an excellent system for the expression of recombinant eukaryotic proteins. Both endogenous and heterologous proteins can be overexpressed in yeast (Phan et al., 2001; Ton and Rao, 2004). Because yeast is easy to manipulate genetically, a strain can be optimized for the expression of a specific protein.
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Expressed protein ligation

European Journal of Biochemistry, 2004
The introduction of noncanonical amino acids and biophysical probes into peptides and proteins, and total or segmental isotopic labelling has the potential to greatly aid the determination of protein structure, function and protein–protein interactions.
Ralf, David   +2 more
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Scalable Transient Protein Expression

2013
Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian cells. Accelerating activity in biotherapeutic drug development, demand for protein-based reagents, vaccine research, and large initiatives in structural and functional studies of proteins have propelled the need to generate moderate to high ...
Henry C, Chiou   +5 more
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STRESS PROTEIN EXPRESSION KINETICS

Annual Review of Biomedical Engineering, 2006
In all organisms there is an elevated synthesis of a select family of “stress proteins” in response to a broad array of environmentally driven stress vectors including elevated or depressed temperature, changes in pH, treatment with many classes of chemicals, ischemia, desiccation, and UV irradiation. The presence of stress proteins, often termed heat
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Protein Identification and Peptide Expression Resolver: Harmonizing Protein Identification with Protein Expression Data

Journal of Proteome Research, 2007
Proteomic discovery platforms generate both peptide expression information and protein identification information. Peptide expression data are used to determine which peptides are differentially expressed between study cohorts, and then these peptides are targeted for protein identification.
Paul, Kearney   +3 more
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Protein Expression

1999
Abstract Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins.
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Adenovirus Proteins And Mhc Expression

1989
Adenoviruses are able to specifically down-regulate the cell surface expression of MHC class I antigens. Most viral serotypes achieve these ends by synthesizing a protein that binds to class I antigens in the endoplasmic reticulum (ER) and impedes the transport of these molecules to the cell surface.
Pääbo, S. ; https://orcid.org/0000-0002-4670-6311   +5 more
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p53 Protein Expression in Leukemias

Acta Oncologica, 1995
p53 protein expression has been investigated by immunohistochemistry in 58 patients with leukemia. Seven of 24 cases with acute myeloid leukemia (AML), 3 of 15 cases with chronic lymphocytic leukemia (CLL), one of 11 cases with chronic myeloid leukemia (CML) and 4 of 8 cases with acute lymphoid leukemia (ALL) had p53 protein expression.
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Gene expression. MicroRNA control of protein expression noise.

Science (New York, N.Y.), 2015
MicroRNAs (miRNAs) repress the expression of many genes in metazoans by accelerating messenger RNA degradation and inhibiting translation, thereby reducing the level of protein. However, miRNAs only slightly reduce the mean expression of most targeted proteins, leading to speculation about their role in the variability, or noise, of protein expression.
Schmiedel, Jörn M   +6 more
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