Results 31 to 40 of about 5,183,482 (306)

Reduced Culture Temperature Differentially Affects Expression and Biophysical Properties of Monoclonal Antibody Variants

Antibodies, 2014
Reduced culture temperature is an increasingly popular practice to improve recombinant protein yields in CHO cells. Recent studies have attributed the enhancement of protein titers at sub-physiological temperatures to increased mRNA levels as well as ...
Megan Mason, Bernadette Sweeney, Katharine Cain, Paul Stephens, Susan T. Sharfstein   +4 more
doaj   +1 more source

DNA polymerase B deficiency is linked to aggressive breast cancer: a comprehensive analysis of gene copy number, mRNA and protein expression in multiple cohorts [PDF]

, 2014
Short arm of chromosome 8 is a hot spot for chromosomal breaks, losses and amplifications in breast cancer. Although such genetic changes may have phenotypic consequences, the identity of candidate gene(s) remains to be clearly defined.
Abayomi, M, Abdel-Fatah, TMA, Agarwal, D, Albarakati, N, Ball, G, Caldas, C, Chan, S, Ellis, IO, Madhusudan, S, Moseley, P, Perry, C, Russell, R   +11 more
core   +1 more source

Differential expression of ADAMTS -1, -4, -5 and TIMP -3 in rat spinal cord at different stages of acute experimental autoimmune encephalomyelitis [PDF]

, 2005
Experimental autoimmune encephalomyelitis (EAE) is an animal model of inflammatory demyelination, a pathological event common to multiple sclerosis (MS). During CNS inflammation there are alterations in the extracellular matrix (ECM).
A.K. Cross, Abbaszzade, Asher, Bandtlow, Barrett, Behi, Brew, Cal, Collins-Racie, D.J. Buttle, G. Haddock, Hartung, Hashimoto, Hill, Inatani, J. Plumb, J. Surr, Jaworski, Kashiwagi, Kashiwagi, Krekoski, Kuno, Kuno, Kurazono, Langton, Lindberg, Lo, Lukes, M.N. Woodroofe, Matthews, Muir, Novak, Novak, Ohgoh, Pangalos, Porter, R.A.D. Bunning, Rosenberg, Sandy, Sasaki, Satoh, Sobel, Somerville, Tang, Teesalu, Toft-Hansen, Vankemmelbeke, Vankemmelbeke, Vazquez, Yamaguchi, Yu, Yuan, Zuo   +52 more
core   +1 more source

Adverse prognostic and predictive significance of low DNA-dependent protein kinase catalytic subunit (DNA-PKcs) expression in early-stage breast cancers [PDF]

, 2014
Background: DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a serine threonine kinase belonging to the PIKK family (phosphoinositide 3-kinase-like-family of protein kinase), is a critical component of the non-homologous end joining (NHEJ ...
AC Wolff, Andrew R. Green, Arvind Arora, B Salles, C Curtis, Christina Perry, Devika Agarwal, Emad Rakha, ET Shinohara, FM Hsu, Graham Ball, HS Lee, HS Lee, Ian O. Ellis, JA Neal, K Meek, K Soderlund Leifler, L Du, LA Loeb, LA Loeb, LM McShane, M Burg van der, Nicola Thompson, Paul Moseley, R Hill, R Sultana, S Medunjanin, SJ Collis, SL Shao, Srinivasan Madhusudan, Stephen Chan, SY Wang, Tarek Abdel-Fatah, TM Abdel-Fatah, TM Abdel-Fatah, TM Abdel-Fatah, U Moll, W Zhuang, X Kong, X Wang, XD Long, Z Hu   +41 more
core   +2 more sources

Connecting protein and mRNA burst distributions for stochastic models of gene expression

, 2011
The intrinsic stochasticity of gene expression can lead to large variability in protein levels for genetically identical cells. Such variability in protein levels can arise from infrequent synthesis of mRNAs which in turn give rise to bursts of protein ...
Andrew T Fenley, Biswas S I, Karmakar R, Mitarai N, Rahul Kulkarni, Tao Jia, van Kampen N G, Vlad Elgart   +7 more
core   +1 more source

Chaperone-assisted protein expression [PDF]

Structure, 1996
I thank the Howard Hughes Medical Institute for a post-doctoral fellowship. I thank Prof Chris Walsh and members of the Walsh group for helpful suggestions.
openaire   +2 more sources

Decreased expression of breast cancer resistance protein in the duodenum in patients with obstructive cholestasis [PDF]

, 2006
Background/Aims: The expression of transporters involved in bile acid homeostasis is differentially regulated during obstructive cholestasis. Since the drug efflux transporter breast cancer resistance protein (BCRP) is known to transport bile acids, we ...
Adachi Y, Brendel C, Christian Zimmermann, Christoph Beglinger, Denk GU, Denson LA, Dietrich CG, Dietrich CG, Ee PL, Frank Lehmann, Freeman LA, Geier A, Gutmann H, Heike Gutmann, Hruz P, Imai Y, Janvilisri T, Jonker JW, Juergen Drewe, Jung D, Jung D, Kamisako T, Klucken J, Li N, Lown KS, Lu TT, Luigi Terracciano, Maliepaard M, Miyake K, Petr Hruz, Plebani M, Popowski K, Schellens JH, Smith M, Stedman CA, Stellaard F, Suzuki M, Taipalensuu J, Tanaka Y, Tanaka Y, Trauner M, Ulrich Beuers, van Herwaarden AE, Volk EL, Wagner M, Xie W, Yu L, Zimmermann C, Zollner G   +48 more
core   +1 more source

Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]

, 2013
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki, Baldi, Beinke, Beinke, Beinke, Beinke, Beinke, Belich, Bouwmeester, Ciechanover, Cohen, Coope, Costa, Dauber, Dumitru, Durocher, Elke MÜHLBERGER, Feng, Gantke, Geisse, George, Gray, Jia, Jia, Julia Janzen, Kabouridis, Kaufman, Lang, Liu, Nicholas A. Morrice, Papoutsopoulou, Pomerantz, Robinson, Roget, Sabrina Boussouf, Salmeron, Salmeron, Savinova, Schlaeger, Schumann, Steven Howell, Steven C. Ley, Thorsten Gantke, Waterfield, Waterfield, Wu, Yang, Zhao   +47 more
core   +1 more source

Producing recombinant proteins in Vibrio natriegens

Microbial Cell Factories
The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system.
Matthew Smith, José Sánchez Hernández, Simon Messing, Nitya Ramakrishnan, Brianna Higgins, Jennifer Mehalko, Shelley Perkins, Vanessa E. Wall, Carissa Grose, Peter H. Frank, Julia Cregger, Phuong Vi Le, Adam Johnson, Mukul Sherekar, Morgan Pagonis, Matt Drew, Min Hong, Stephanie R. T. Widmeyer, John-Paul Denson, Kelly Snead, Ivy Poon, Timothy Waybright, Allison Champagne, Dominic Esposito, Jane Jones, Troy Taylor, William Gillette   +26 more
doaj   +1 more source

Survival Outcomes and Complications Among Canadian Children With Retinoblastoma: A Population‐Based Report From CYP‐C

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Purpose Retinoblastoma (RB) is the most common pediatric ocular cancer, yet population‐based data on survival and risk factors remain limited. This study aimed to describe survival in a large national RB cohort and identify predictors of death and complications.
Samuel Sassine, Ofélie Trudeau‐Ferrin, Caroline Laverdière, Patrick Hamel, Rosanne Superstein, Bénédicte Koukoui, Carole Provost, Paul C. Nathan, Vijay Ramaswamy, Furqan Shaikh, Brenda Gallie, Ashwin Mallipatna, Stephanie Kletke, Helen Dimaras, Hallie Coltin   +14 more
wiley   +1 more source