Results 11 to 20 of about 5,183,482 (306)

Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling

Nature Communications, 2021
Understanding the infection parameters and host responses against SARS-CoV-2 require data from large cohorts using standardized methods. Here, the authors optimize a serum ELISA protocol that has minimal cross-reactivity and flexible sample collection ...
Carleen Klumpp-Thomas, Heather Kalish, Matthew Drew, Sally Hunsberger, Kelly Snead, Michael P. Fay, Jennifer Mehalko, Anandakumar Shunmugavel, Vanessa Wall, Peter Frank, John-Paul Denson, Min Hong, Gulcin Gulten, Simon Messing, Jennifer Hicks, Sam Michael, William Gillette, Matthew D. Hall, Matthew J. Memoli, Dominic Esposito, Kaitlyn Sadtler   +20 more
doaj   +1 more source

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology, 2021
At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang, Tian-Yun Wang   +4 more
doaj   +1 more source

Autoinduction of Protein Expression [PDF]

Current Protocols in Protein Science, 2009
AbstractThis unit contains protocols for the use of lactose‐derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies.
Brian G, Fox, Paul G, Blommel
openaire   +2 more sources

GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

BMC Biotechnology, 2020
Background Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and ...
Jana Neuhold, Katharina Radakovics, Anita Lehner, Florian Weissmann, Maria Queralt Garcia, Mari Carmen Romero, Nicholas S. Berrow, Peggy Stolt-Bergner   +7 more
doaj   +1 more source

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu, Hui-Ning Liu, Wei-Hua Dong, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang, Tian-Yun Wang   +7 more
doaj   +1 more source

The Expression Levels of XLF and Mutant P53 Are Inversely Correlated in Head and Neck Cancer Cells. [PDF]

, 2016
XRCC4-like factor (XLF), also known as Cernunnos, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks.
Chen, Wei, Feng, Sizhe, Guo, Mian, Hu, Shen, Liang, Guobiao, Messadi, Diana, Rabii, Ramin, Song, Chenxi, Wei, Xuezhong   +8 more
core   +2 more sources

Oestrogen receptor alpha in pulmonary hypertension [PDF]

, 2015
Aims Pulmonary arterial hypertension (PAH) occurs more frequently in women with mutations in bone morphogenetic protein receptor type 2 (BMPR2) and dysfunctional BMPR2 signalling underpinning heritable PAH.
Audrey F. Wright, Austin, Benza, Chambliss, Dempsie, Dempsie, Eddahibi, Huang, Humbert, Jala, Kawut, Keegan, Kirsty Mair, Lahm, Lindberg, Long, Long, Lynn Loughlin, Ma, Machado, MacLean, Mair, Margaret Nilsen, Margaret R. Maclean, Marie-Ann Ewart, Marino, Massaro, Massaro, Matori, Matsumoto, Mcgoon, Mendelsohn, Meyer, Meyer, Morani, Nadadur, Nilsson, Pattarozzi, Pedram, Pedram, Rajkumar, Roberts, Rubanyi, Ryu, Shapiro, Takahashi, Umar, Ventetuolo, White, White, White, Wu, Yang, Yvonne Dempsie   +53 more
core   +4 more sources

Protein Expression and Purification [PDF]

Klinicka onkologie, 2014
Production of recombinant proteins is essential for many applications in both basic research and also in medicine, where recombinant proteins are used as pharmaceuticals. This review summarizes procedures involved in recombinant protein expression and purification, including molecular cloning of target genes into expression vectors, selection of the ...
E, Růčková, P, Müller, B, Vojtěšek
openaire   +2 more sources

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR [PDF]

, 2016
Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression regulation. To study lncRNAs in cancer, we generated expression profiles for 1707 human lncRNAs in the NCI60 cancer cell line
Derveaux, Stefaan, Hellemans, Jan, Lefever, Steve, Mestdagh, Pieter, Vandesompele, Jo, Volders, Pieter-Jan   +5 more
core   +2 more sources

Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) deficiency is linked to aggressive breast cancer and predicts response to adjuvant therapy [PDF]

, 2013
Uracil in DNA is an important cause of mutagenesis. SMUG1 is a uracil DNA glycosylase that removes uracil through base excision repair. SMUG1 also processes radiation induced oxidative base damage as well as 5-fluorouracil incorporated into DNA during
Abdel-Fatah, TMA, Agarwal, D, Albarakati, N, Ball, G, Bowell, L, Chan, S, Ellis, IO, Hawkes, C, Madhusudan, S, Moseley, P   +9 more
core   +1 more source