Results 1 to 10 of about 5,183,482 (306)
Expressed Protein Selenoester Ligation [PDF]
Angewandte Chemie, 2022 AbstractHerein, we describe the development and application of a novel expressed protein selenoester ligation (EPSL) methodology for the one‐pot semi‐synthesis of modified proteins. EPSL harnesses the rapid kinetics of ligation reactions between modified synthetic selenopeptides and protein aryl selenoesters (generated from expressed intein fusion ...
Sameer S. Kulkarni +10 more
openaire +5 more sources
Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins
Nature Communications, 2021 RaPID (Random non-standard Peptides Integrated Discovery) enables discovery of small macrocyclic peptides binding desired targets. Here, the authors propose lasso-grafting: the RaPID-derived peptides are implanted onto diverse proteins and maintain both ...
Emiko Mihara +12 more
doaj +1 more source
Structural mechanism of laminin recognition by integrin
Nature Communications, 2021 Recognition of laminin by integrin receptors mediates epithelial cell adhesion to basement membrane. Here, the structures of the α6β1 integrin alone and in complex with three-chain laminin-511 fragment reveal the laminin-integrin interface in molecular ...
Takao Arimori +7 more
doaj +1 more source
Artificial environments for the co-translational stabilization of cell-free expressed proteins [PDF]
, 2013 An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of ...
Bernhard, Frank +3 more
core +10 more sources
Scientific Reports, 2022 Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans.
Laudine M. C. Petralia +10 more
doaj +1 more source
Molecular Therapy: Oncolytics, 2023 CD47 and its receptor signal regulatory protein α (SIRPα) act as a dominant antiphagocytic, “don’t eat me” signal. Recent studies reveal CD24 as a novel target for cancer immunotherapy by macrophages in ovarian cancer and breast cancer.
Yun Yang +10 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 Chinese hamster ovary (CHO) cells are currently the most widely used host cells for recombinant therapeutic protein (RTP) production. Currently, the RTP yields need to increase further to meet the market needs and reduce costs.
Qin Li +8 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression
Mian Wei +4 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li +7 more
doaj +1 more source
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells
Frontiers in Bioengineering and Biotechnology, 2021 As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells.
Huan-Yu Zhang +5 more
doaj +1 more source