Results 111 to 120 of about 251,250 (306)
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting. [PDF]
Proc Natl Acad Sci U S A, 2020 Han S +5 more
europepmc +1 more source
Mapping Interactions of Microbial Metabolites with Human G-Protein-Coupled Receptors [PDF]
, 2019 Dominic A. Colosimo +9 more
openalex +1 more source
β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1
Molecular Oncology, EarlyView.Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero +8 more
wiley +1 more source
Mapping RNA-Protein Interactions with Subcellular Resolution Using Colocalization CLIP [PDF]
, 2023 Soon Yi +3 more
openalex +1 more source
Molecular Oncology, EarlyView.AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou +5 more
wiley +1 more source
Next-Generation Protein–Ligand Interaction Networks: APEX as a Powerful Technology
ProteomesPeroxidases are essential enzymes that catalyze redox reactions, with wide-ranging biological implications. Among these, an enhanced ascorbate peroxidase (APEX) has emerged as a valuable tool for studying intricate intracellular events with ...
José Miguel Quintero-Ferrer +7 more
doaj +1 more source
Physical and Functional Mapping of the Replication Protein A Interaction Domain of the Werner and Bloom Syndrome Helicases [PDF]
, 2005 Kevin M. Doherty +8 more
openalex +1 more source
Molecular Oncology, EarlyView.This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan +9 more
wiley +1 more source
Novel Technology for Protein-Protein Interaction-based Targeted Drug Discovery
Journal of Analytical Science and Technology, 2011 We have developed a simple but highly efficient in-cell protein-protein interaction (PPI) discovery system based on the translocation properties of protein kinase C- and its C1a domain in live cells.
Jung Me Hwang +7 more
doaj