Results 331 to 340 of about 1,237,822 (372)

Mapping protein–protein interactions by mass spectrometry

Current Opinion in Biotechnology, 2006
Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction ...
Julian Vasilescu, Daniel Figeys
openaire   +3 more sources

Deconvolution of Targeted Protein–Protein Interaction Maps

Journal of Proteome Research, 2012
Current proteomic techniques allow researchers to analyze chosen biological pathways or an ensemble of related protein complexes at a global level via the measure of physical protein-protein interactions by affinity purification mass spectrometry (AP-MS). Such experiments yield information-rich but complex interaction maps whose unbiased interpretation
Jacques Colinge, Alexey Stukalov, Giulio Superti-Furga   +2 more
openaire   +3 more sources

The protein–protein interaction map of Helicobacter pylori

Nature, 2001
With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function. As elementary constituents of cellular protein complexes and pathways, protein-protein interactions are key ...
Luc Selig, Véronique Battaglia, Céline Reverdy, Jean-Christophe Rain, Gerlinde Lenzen, Hilde De Reuse, Vincent Schächter, Y. Chemama, Fabien Petel, Jérôme Wojcik, Agnès Labigne, Stéphane Simon, Pierre Legrain   +12 more
openaire   +3 more sources

High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.

Methods, 2001
Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have ...
A. J. Walhout, M. Vidal
semanticscholar   +1 more source

Novel approaches to map protein interactions

Current Opinion in Biotechnology, 2003
Although we now have the sequence of the human genome at hand, we face the challenge of assigning function to the identified genes. Genes usually ascribe their function through proteins, and the role of proteins is to interact with other molecules. Therefore, if we could map the interactions of proteins we would be able to understand protein function ...
openaire   +3 more sources

Protein-interaction mapping in search of effective drug targets.

Bioessays, 2005
Signaling complexes and networks are being intensely studied in an attempt to discover pathways that are amenable to therapeutic intervention. A challenge in this search is to understand the effect that the modulation of a target will have on the overall
Amitabha Chaudhuri, J. Chant
semanticscholar   +1 more source

A Protein Interaction Map of Drosophila melanogaster

Science, 2003
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 ...
C. E. Ooi, John Chant, Steven Allen Gold, Y. Lazovatsky, James R. Knight, G. Vijayadamodar, Pascale Pochart, E. Voss, Julia Williams, H. Machineni, B. Zerhusen, Ying Li, E. Bickelhaupt, Kevin P. White, B. Kuang, E. Vitols, Rachel D. Malcolm, N. Aanensen, N. Ioime, Y. L. Hao, Cory Brouwer, Clement A. Stanyon, S. Burgess, Loic Giot, L. McDaniel, K. Neurath, Richard A. Shimkets, Joel S. Bader, K. Furtak, Z. Varrone, F. Spriggs, A. Collis, E. Stimpson, Michael P. McKenna, Michael S. Braverman, Jonathan M. Rothberg, Brian C. Godwin, Amitabha Chaudhuri, M. Welsh, Yong Kong, M. Leach, R. Renzulli, Russell L. Finley, A. DaSilva, Jinhui Zhong, M. Minto, M. Agee, Thomas P. Jarvie, S. Carrolla   +48 more
openaire   +3 more sources

Detecting protein-protein interaction based on protein fragment complementation assay.

Current protein and peptide science, 2020
Proteins are the most critical executive molecules by responding to the instructions stored in the genetic materials in any form of life. More frequently, proteins do their jobs by acting as a roleplayer that interacts with other protein(s), which is ...
Tianwen Wang, Ningning Yang, Chen Liang, Hongjv Xu, Yafei An, Sha Xiao, Mengyuan Zheng, Lu Liu, Gaozhan Wang, L. Nie   +9 more
semanticscholar   +1 more source

Protein interface pharmacophore mapping tools for small molecule protein: protein interaction inhibitor discovery.

Current Topics in Medicinal Chemistry, 2013
Protein:protein interactions are becoming increasingly significant as potential drug targets; however, the rational identification of small molecule inhibitors of such interactions remains a challenge.
A. Voet, E. F. Banwell, K. Sahu, J. Heddle, Kam Y. J. Zhang   +4 more
semanticscholar   +1 more source

Mapping Protein-RNA Interactions by CLIP

Materials and Methods, 2011
One of the challenges of systems biology is to understand how the interplay of individual cellular components gives rise to cellular behaviors such as cell division, apoptosis and differentiation. Generally, the expression of genes encoding proteins that are part of the same pathway is regulated in a coordinated manner, either at the level of ...
Gorges Martin, Andreas R. Gruber, Mihaela Zavolan   +2 more
openaire   +2 more sources