Results 341 to 350 of about 1,237,822 (372)

Human signaling pathways analyzed by protein interaction mapping

, 2005
Combining protein–protein interaction mapping with cellular assays performed for functional validation provides biological support to large-scale protein interaction datasets. This strategy was recently developed for specific signal transduction pathways
F. Colland, P. Legrain
semanticscholar   +1 more source

Construction and Prediction of Protein-Protein Interaction Maps

2002
With the completion of full genome sequence for several model organisms, new approaches are emerging to comprehensively characterize the function of gene products. In these so-called functional proteomics approaches, large-scale assays on the complete set of proteins of a given organism — the proteome — enable the study of the function of proteins in ...
openaire   +3 more sources

Transcriptome-Wide Mapping of Protein–RNA Interactions

2020
RNA and RNA-binding proteins (RBPs) control multiple biological processes. The spatial and temporal arrangement of RNAs and RBPs underlies the delicate regulation of these processes. The strategy called CLIP-seq (cross-linking and immunoprecipitation) has been developed to capture endogenous protein-RNA interactions with UV cross-linking followed by ...
Xiaohua Shen, Xianju Bi
openaire   +3 more sources

Scaffolding and protein interactions in MAP kinase modules

Cell Calcium, 1999
MAP kinases are a family of protein kinases that are ubiquitously expressed and play roles in most signal transduction pathways. They are activated within protein kinase cascades consisting of at least three kinases acting in series. In many, if not all cases, the three-kinase cascade, conveniently referred to as a MAP kinase module, is organized on ...
Mahesh Karandikar, Melanie H. Cobb
openaire   +3 more sources

Proteolytic DNA for Mapping Protein−DNA Interactions

Biochemistry, 2002
We describe a technique to determine sites on proteins involved in protein-DNA interactions. DNA was synthesized via polymerase chain reaction (PCR) to produce four polynucleotide products with phosphorothioate nucleotides at the A, T, G, or C residues.
Brian D. Schmidt, Claude F. Meares
openaire   +3 more sources

Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.

Journal of Proteome Research, 2011
Mortality attributable to infection with methicillin-resistant Staphylococcus aureus (MRSA) has now overtaken the death rate for AIDS in the United States, and advances in research are urgently needed to address this challenge.
A. Cherkasov, Michael Hsing, R. Zoraghi, L. Foster, R. See, N. Stoynov, Jihong Jiang, S. Kaur, Tian Lian, Linda Jackson, H. Gong, Richard D. Swayze, E. Amandoron, F. Hormozdiari, Phuong Dao, Cenk Sahinalp, O. Santos-Filho, Peter Axerio-Cilies, K. Byler, W. McMaster, R. Brunham, B. Finlay, N. Reiner   +22 more
semanticscholar   +1 more source

Progress and potential of Drosophila protein interaction maps

Pharmacogenomics, 2000
Protein-protein interactions mediate many important cellular processes and are central to the mechanisms by which most proteins function. Charting the interactions among the proteins involved in a process has been an essential step in characterising the function of proteins and pathways.
Clement A. Stanyon, Russell L. Finley
openaire   +3 more sources

Deciphering complex protein interaction kinetics using Interaction Map

Biochemical and Biophysical Research Communications, 2012
Cellular receptor systems are expected to present complex ligand interaction patterns that cannot be evaluated assuming a simple one ligand:one receptor interaction model. We have previously evaluated heterogeneous interactions using an alternative method to regression analysis, called Interaction Map (IM).
Magnus Malmqvist, Hanna Björkelund, Danièle Altschuh, Laurence Choulier, John Strandgård, Karl Andersson   +5 more
openaire   +2 more sources

Mapping the Yeast Protein Interaction Network

Science Signaling, 2008
To move beyond genes to an understanding of networks, it is necessary to track protein-protein interactions in vivo. Tarassov et al . have used protein-fragment complementation assays, which are based on reassembly of two domains of the enzyme dihydrofolate reductase that have been fused to the proteins of interest,
openaire   +2 more sources

Multiplex mapping of protein-protein interaction interfaces

AbstractWe describe peptide mapping throughSplitAntibioticResistanceComplementation (SpARC-map), a method to identify the probable interface between two interacting proteins. Our method is based on in vivo affinity selection inside a bacterial host, and uses high throughput DNA sequencing results to infer the location of protein-protein interaction ...
Jingxuan He, Ling-Nan Zou, Vidhi Pareek, Stephen J. Benkovic   +3 more
openaire   +2 more sources