Results 311 to 320 of about 2,191,855 (361)

A-kinase anchoring proteins: protein kinase A and beyond

Current Opinion in Cell Biology, 2000
Compartmentalization of kinases and phosphatases is a key determinant in the specificity of second messenger mediated signaling events. Localization of the cAMP-dependent protein kinase (PKA) and other signaling enzymes is mediated by interaction with A-kinase anchoring proteins (AKAPs).
A S, Edwards, J D, Scott
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A Multiplexed Protein Kinase Assay

ChemBioChem, 2007
AbstractWe report a novel protein kinase assay designed for high‐throughput detection of one or many kinases in a complex mixture. A solution‐phase phosphorylation reaction is performed on 900 different peptide substrates, each covalently linked to an oligonucleotide tag.
Melissa D, Shults, Igor A, Kozlov, Nicholas, Nelson, Bahram G, Kermani, Peter C, Melnyk, Veronika, Shevchenko, Anu, Srinivasan, Joseph, Musmacker, John P, Hachmann, David L, Barker, Michal, Lebl, Chanfeng, Zhao   +11 more
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A new family of protein kinases— The mitochondrial protein kinases

Advances in Enzyme Regulation, 1995
Molecular cloning has provided evidence for a new family of protein kinases in eukaryotic cells. These kinases show no sequence similarity with other eukaryotic protein kinases, but are related by sequence to the histidine protein kinases found in prokaryotes. These protein kinases, responsible for phosphorylation and inactivation of the branched-chain
R A, Harris, K M, Popov, Y, Zhao, N Y, Kedishvili, Y, Shimomura, D W, Crabb   +5 more
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A-kinase anchoring protein targeting of protein kinase A in the heart

Journal of Molecular and Cellular Cardiology, 2004
There is increasing evidence that subcellular targeting of signaling molecules is an important means of regulating the protein kinase A (PKA) pathway. Subcellular organization of the signaling molecules in the PKA pathway insures that a signal initiated at the receptor level is transferred efficiently to a PKA substrate eliciting some cellular response.
Mary L, Ruehr, Mary A, Russell, Meredith, Bond   +2 more
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Method for Simultaneous Detection of Protein Kinase A, Protein Kinase C, Protein Tyrosine Kinase, and Calmodulin-Dependent Protein Kinase Activities

Analytical Biochemistry, 1993
We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of v-src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and [gamma-32P]ATP, and the phosphorylated proteins are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
H, Fukazawa, P M, Li, S, Mizuno, Y, Uehara   +3 more
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Protein Kinase D Is a Downstream Target of Protein Kinase Cθ

Biochemical and Biophysical Research Communications, 2002
Protein kinase D (PKD/PKCmu immunoprecipitated from either COS-7 cells or Jurkat T lymphocytes transiently transfected with a constitutively active mutant of PKCtheta AE (PKCthetaAE) exhibited a marked increase in basal activity. In contrast, coexpression of constitutively active mutant of PKCzeta does not induce PKD activation in both types of cells ...
Jingzhen, Yuan, David, Bae, Doreen, Cantrell, Andre E, Nel, Enrique, Rozengurt   +4 more
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Regulation of a Mitogen-Activated Protein Kinase Kinase Kinase, MLTK by PKN

Journal of Biochemistry, 2003
PKNalpha is a fatty acid- and Rho-activated serine/threonine protein kinase having a catalytic domain homologous to members of the protein kinase C family. Recently it was reported that PKNalpha is involved in the p38 mitogen-activated protein kinase (MAPK) signaling pathway.
Mikiko, Takahashi, Yusuke, Gotoh, Takayuki, Isagawa, Tamako, Nishimura, Emiko, Goyama, Hon-Song, Kim, Hideyuki, Mukai, Yoshitaka, Ono   +7 more
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Protein kinase C — a family affair

Molecular and Cellular Endocrinology, 1989
The structural analysis of protein kinase C has led to the identification of a family of related gene products. This family of kinases consists of six unique genes that give rise to at least seven polypeptides. The high degree of conservation and the differential distribution of these mRNAs/proteins suggest that they perform distinct functions in vivo.
P J, Parker, G, Kour, R M, Marais, F, Mitchell, C, Pears, D, Schaap, S, Stabel, C, Webster   +7 more
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