Results 211 to 220 of about 1,706,932 (293)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

The extraordinary satellitome diversity of freshwater crayfish: a driver of genome evolution. [PDF]

Mob DNA
Bonassin L, Boštjančić LL, Rutz C, Francesconi C, Schardt L, Baranski D, Greve C, Pârvulescu L, Besendorfer V, Mlinarec J, Maguire I, Theissinger K, Lecompte O.   +12 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Distinct evolutionary patterns of endemic and emerging parvoviruses and the origin of a new pandemic virus. [PDF]

Proc Natl Acad Sci U S A
López-Astacio RA, Wasik BR, Lee H, Voorhees IEH, Weichert WS, Adu OF, Goodman LB, Hafenstein SL, Truyen U, Parrish CR.   +9 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Data storage and retrieval with unnatural proteins expressed via E. coli. [PDF]

Nat Commun
Zhou Y, Ng CCA, Liu C, Tam WM, Lau FCM, Yao ZP.   +5 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Genome modelling and design across all domains of life with Evo 2. [PDF]

Nature
Brixi G, Durrant MG, Ku J, Naghipourfar M, Poli M, Sun G, Brockman G, Chang D, Fanton A, Gonzalez GA, King SH, Li DB, Merchant AT, Nguyen E, Ricci-Tam C, Romero DW, Schmok JC, Taghibakhshi A, Vorontsov A, Yang B, Deng M, Gorton L, Nguyen N, Wang NK, Pearce MT, Simon E, Adams E, Amador ZJ, Ashley EA, Baccus SA, Dai H, Dillmann S, Ermon S, Guo D, Herschl MH, Ilango R, Janik K, Lu AX, Mehta R, Mofrad MRK, Ng MY, Pannu J, Ré C, St John J, Sullivan J, Tey J, Viggiano B, Zhu K, Zynda G, Balsam D, Collison P, Costa AB, Hernandez-Boussard T, Ho E, Liu MY, McGrath T, Powell K, Pinglay S, Burke DP, Goodarzi H, Hsu PD, Hie BL.   +61 more
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Lifestyle-associated variation in type IV secretion systems between phytopathogenic and environmental <i>Ralstonia</i>. [PDF]

Microb Genom
Cowell TC, Cope-Arguello ML, Shin GY, Bogdanove AJ, Carpenter SCD, Nahar K, Saha A, Lowe-Power TM.   +7 more
europepmc   +1 more source