Results 71 to 80 of about 11,322,924 (398)

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

eLife, 2016
Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S.
Colin Echeverría Aitken, Petra Beznosková, Vladislava Vlčkova, Wen-Ling Chiu, Fujun Zhou, Leoš Shivaya Valášek, Alan G Hinnebusch, Jon R Lorsch   +7 more
doaj   +1 more source

Dietary Methionine Restriction Regulates Liver Protein Synthesis and Gene Expression Independently of Eukaryotic Initiation Factor 2 Phosphorylation in Mice [PDF]

, 2017
Background: The phosphorylation of eukaryotic initiation factor 2 (p-eIF2) during dietary amino acid insufficiency reduces protein synthesis and alters gene expression via the integrated stress response (ISR).Objective: We explored whether a Met ...
Anthony, Tracy G., Bargoud, Albert R., Gettys, Thomas W., Hamilton, Karyn L., Jonsson, William O., Kimball, Scot R., Miller, Benjamin F., Mirek, Emily T., Peelor, Frederick F., III, Pettit, Ashley P., Wang, Yongping, Wek, Ronald C.   +11 more
core   +1 more source

Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men.

The journals of gerontology. Series A, Biological sciences and medical sciences, 2014
BACKGROUND Adequate protein ingestion-mediated stimulation of myofibrillar protein synthesis (MPS) is required to maintain skeletal muscle mass. It is currently unknown what per meal protein intake is required to maximally stimulate the response in older
D. Moore, T. Churchward-Venne, Oliver C. Witard, L. Breen, N. Burd, K. Tipton, Stuart M Phillips   +6 more
semanticscholar   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

eIF3a Regulation of NHEJ Repair Protein Synthesis and Cellular Response to Ionizing Radiation

Frontiers in Cell and Developmental Biology, 2020
Translation initiation in protein synthesis regulated by eukaryotic initiation factors (eIFs) is a crucial step in controlling gene expression. eIF3a has been shown to regulate protein synthesis and cellular response to treatments by anticancer agents ...
Rima Tumia, Chao J. Wang, Tianhan Dong, Shijie Ma, Jenny Beebe, Juan Chen, Zizheng Dong, Jing-Yuan Liu, Jian-Ting Zhang, Jian-Ting Zhang   +9 more
doaj   +1 more source

Protein synthesis at synaptic sites on dendrites [PDF]

, 2001
Studies over the past 20 years have revealed that gene expression in neurons is carried out by a distributed network of translational machinery. One component of this network is localized in dendrites, where polyribosomes and associated membranous ...
Schuman, Erin M., Steward, Oswald
core   +1 more source

Partial restoration of protein synthesis rates by the small molecule ISRIB prevents neurodegeneration without pancreatic toxicity

Cell Death and Disease, 2015
Activation of the PERK branch of the unfolded protein response (UPR) in response to protein misfolding within the endoplasmic reticulum (ER) results in the transient repression of protein synthesis, mediated by the phosphorylation of the alpha subunit of
Mark Halliday, H. Radford, Yusuke Sekine, Julie A. Moreno, Nicholas C. Verity, J. L. Quesne, C. Ortori, David A. Barrett, Christophe Fromont, Peter M. Fischer, H. Harding, David Ron, G. Mallucci   +12 more
semanticscholar   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

γ effects identify preferentially populated rotamers of CH₂F groups: side-chain conformations of fluorinated valine analogues in a protein [PDF]

Magnetic Resonance
Using cell-free protein synthesis, the protein G B1 domain (GB1) was prepared with uniform high-level substitution of valine by (2S,3S)-4-fluorovaline, (2S,3R)-4-fluorovaline or 4,4'-difluorovaline.
E. H. Abdelkader, N. F. Chilton, N. F. Chilton, A. Maleckis, G. Otting   +4 more
doaj   +1 more source

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

ChemBioChem, 2015
From its start as a small‐scale in vitro system to study fundamental translation processes, cell‐free protein synthesis quickly rose to become a potent platform for the high‐yield production of proteins.
Anne Zemella, Lena Thoring, Christian Hoffmeister, S. Kubick   +3 more
semanticscholar   +1 more source