Results 21 to 30 of about 10,718,710 (345)
Ultra-sensitive fluorescent proteins for imaging neuronal activity
Nature, 2013 Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured ...
Tsai-Wen Chen +12 more
semanticscholar +1 more source
Haematologica, 2017 Factor VIII C-domains are believed to have specific functions in cofactor activity and in interactions with von Willebrand factor. We have previously shown that factor VIII is co-targeted with von Willebrand factor to the Weibel-Palade bodies in blood ...
Eduard H.T.M. Ebberink +7 more
doaj +1 more source
DOCKGROUND Membrane Protein-Protein Set [PDF]
PLOS ONE, 2021 ABSTRACTMembrane proteins play essential role in cellular mechanisms. Despite that and the major progress in experimental structure determination, they are still significantly underrepresented in Protein Data Bank. Thus, computational approaches to protein structure determination, which are important in general, are especially valuable in the case of ...
Ian Kotthoff +2 more
openaire +4 more sources
Exploring the kinetic selectivity of drugs targeting the β1‐adrenoceptor
Pharmacology Research & Perspectives, 2022 In this study, we report the β1‐adrenoceptor binding kinetics of several clinically relevant β1/2‐adrenoceptor (β1/2AR) agonists and antagonists. [3H]‐DHA was used to label CHO‐β1AR for binding studies. The kinetics of ligand binding was assessed using a
David A. Sykes +5 more
doaj +1 more source
Scientific Reports, 2021 Increasing evidence has indicated that adult neurogenesis contributes to brain plasticity, although function of new neurons is still under debate. In opossums, we performed an olfactory-guided behavior task and examined the association between olfactory ...
Beata Tepper +5 more
doaj +1 more source
Aquaporin Protein-Protein Interactions [PDF]
International Journal of Molecular Sciences, 2017 Aquaporins are tetrameric membrane-bound channels that facilitate transport of water and other small solutes across cell membranes. In eukaryotes, they are frequently regulated by gating or trafficking, allowing for the cell to control membrane permeability in a specific manner.
Jennifer Roche +1 more
openaire +2 more sources
Interfacial Protein–Protein Associations [PDF]
Biomacromolecules, 2013 While traditional models of protein adsorption focus primarily on direct protein-surface interactions, recent findings suggest that protein-protein interactions may play a central role. Using high-throughput intermolecular resonance energy transfer (RET) tracking, we directly observed dynamic, protein-protein associations of bovine serum albumin on ...
Blake B, Langdon +3 more
openaire +2 more sources
Haematologica, 2016 It has been proposed that von Willebrand factor might affect factor VIII immunogenicity by reducing factor VIII uptake by antigen presenting cells. Here we investigate the interaction of recombinant von Willebrand factor with immature monocyte-derived ...
Nicoletta Sorvillo +8 more
doaj +1 more source
ESC Heart Failure, 2020 Aims Cardiac transthyretin amyloidosis (ATTR‐CM) is a progressive and fatal condition. Prognosis can be determined at diagnosis according to the National Amyloidosis Centre (NAC) transthyretin amyloidosis (ATTR) stage.
Steven Law +14 more
doaj +1 more source
14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains
Communications Biology, 2021 Pohl et al. investigated the structural basis of Nedd4-2 regulation by 14-3-3 and found that phosphorylated Ser342 and Ser448 are the main residues that facilitate 14-3-3 binding to Nedd4-2.
Pavel Pohl +4 more
doaj +1 more source