Results 291 to 300 of about 13,117,352 (339)

DISCOVERING PROTEIN–PROTEIN INTERACTIONS

Journal of Bioinformatics and Computational Biology, 2004
The ongoing genomics and proteomics efforts have helped identify many new genes and proteins in living organisms. However, simply knowing the existence of genes and proteins does not tell us much about the biological processes in which they participate. Many major biological processes are controlled by protein interaction networks.
See-Kiong Ng, Soon-Heng Tan
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Protein Phosphorylation and Protein-Protein Interactions

2002
Intracellular signaling is the series of events which translates the specific message of circulating ligands into a particular biological response in target cells. The first step in hormone signaling involves the interaction between a ligand and its cognate receptor (membrane or nuclear), which in turn induces stoichiometric and conformational changes ...
Paul A. Kelly, Vincent Goffin
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Protein–protein interactions of KChIP proteins and Kv4.2

Biochemical and Biophysical Research Communications, 2004
To prove heteromeric assembly of KChIP proteins, the present study is carried out. The results of chemical crosslinking and pull down assay revealed that KChIP1, KChIP2.1, and KChIP2.2 could form homo- as well as hetero-oligomer, and this oligomerization exhibited a Ca(2+)-dependent manner. Moreover, homomeric and heteromeric assembly of KChIPs did not
Ching-Ping Cheng, Long-Sen Chang, Ya-Ling Lin, Chia-Yi Chen   +3 more
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Protein recruitment systems for the analysis of protein–protein interactions

Biochemical Pharmacology, 2000
The yeast Saccharomyces cerevisiae serves as an excellent genetic tool for the analysis of protein +/- protein interactions. The most common system, used to date, is the two-hybrid system. Although proven very powerful, the two-hybrid system exhibits several inherent problems and limitations.
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Osmolytes and Protein–Protein Interactions

Journal of the American Chemical Society, 2018
Cells survive fluctuations in osmolality by accumulating and depleting highly soluble, usually neutral, small organic compounds. Natural selection has converged on a small set of such molecules, called osmolytes. The biophysical characterization of osmolytes, with respect to proteins, has centered on tertiary structure stability.
Amy E. Rydeen, Eric M. Brustad, Gary J. Pielak   +2 more
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Hsp70 proteins in protein translocation

2001
Publisher Summary Hsp70 proteins are essential components of the protein translocation systems of the endoplasmic reticulum (ER) and mitochondria. The role of Hsp70s in protein translocation is not confined to the ER and mitochondria. For most proteins of the ER, translocation is coupled with protein synthesis.
Ryan, Michael T., Pfanner, Nikolaus
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Nature, 1970
U. Laemmli
semanticscholar   +1 more source

Protein Arrays: Labeling the Protein and Probing the Array for Protein-Protein Interactions

Cold Spring Harbor Protocols, 2007
INTRODUCTIONTo detect protein-protein interactions, a protein microarray can be incubated with a fluorescently labeled protein. After the array is washed, stable interactions can be identified by scanning the slide for fluorescent spots. This protocol uses cyanine-3 (Cy3) or cyanine-5 (Cy5) monofunctionally activated dyes to label the probe proteins.
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Protein–Protein Interactions: Protein interactions contribute to protein function

Trends in Genetics, 2002
Protein–Protein Interactions (A Molecular Cloning Manual) edited by Erica GolemisCold Spring Harbor Laboratory Press, 2001. £100.00 pbk (600 pages)ISBN 0 879 69628 1Proteins control and mediate many of the biological activities of cells. Hence, to gain an understanding of cellular function, the function of every protein must be understood, both in ...
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All-atom empirical potential for molecular modeling and dynamics studies of proteins.

Journal of Physical Chemistry B, 1998
Alexander D. MacKerell, D. Bashford, M. Bellott, R. Dunbrack, J. Evanseck, M. Field, S. Fischer, J. Gao, H. Guo, S. Ha, D. Joseph-McCarthy, L. Kuchnir, K. Kuczera, F. Lau, C. Mattos, S. Michnick, T. Ngo, D. Nguyen, B. Prodhom, W. Reiher, B. Roux, M. Schlenkrich, J. Smith, R. Stote, J. Straub, M. Watanabe, J. Wiórkiewicz-Kuczera, D. Yin, M. Karplus   +28 more
semanticscholar   +1 more source