Microinjection of β-glucan Into Larval Zebrafish (Danio rerio) for the Assessment of a Trained-Like Immunity Phenotype [PDF]
The innate immune system can remember previous inflammatory insults, enabling long-term heightened responsiveness to secondary immune challenges in a process termed "trained immunity." Trained innate immune cells undergo metabolic and epigenetic ...
Hall, Christopher J +2 more
core +1 more source
Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue. [PDF]
Stem and progenitor cells isolated from human limbal tissue can be cultured in vitro as spheres. These spheres have potential for use as transplantable elements for the repopulation of corneal tissue ( Mathan et al., 2016 ).
Ismail, Salim +3 more
core +1 more source
Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids [PDF]
Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades.
Bag, Pushan, +7 more
core +1 more source
A Protocol to Retrieve and Curate Spatial and Climatic Data from Online Biodiversity Databases Using R [PDF]
Ecological and evolutionary studies often require high quality biodiversity data. This information is readily available through the many online databases that have compiled biodiversity data from herbaria, museums, and human observations.
Nagore Medina +5 more
core +1 more source
Interactions amongst different processes in concurrent software are governed by a protocol. The blocking I/O operations involved in a protocol may temporarily suspend the execution of some processes in an application. Scheduling consists of the allocation of available processors to the appropriate non-suspended processes in an application, such that ...
K.P.C. Dokter (Kasper) +1 more
openaire +2 more sources
Blockchain Protocols and Edge Computing Targeting Industry 5.0 Needs
“Industry 5.0” is the latest industrial revolution. A variety of cutting-edge technologies, including artificial intelligence, the Internet of Things (IoT), and others, come together to form it.
Miguel Oliveira +4 more
doaj +1 more source
A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS) [PDF]
Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are ...
Arslan, Tugba Sezgin +2 more
core +1 more source
Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells [PDF]
peer-reviewedEnsifer adhaerens OV14, a soil borne alpha-proteobacteria of the Rhizobiaceae family, fortifies the novel plant transformation technology platform termed ‘Ensifer-mediated transformation’ (EMT).
Fiona Doohan +5 more
core +1 more source
Quantification of the humidity effect on HR by Ion leakage assay. [PDF]
We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes.
Dong, Xinnian, Mwimba, Musoki
core +1 more source
Smart Contract-Based Secure Model for Miner Registration and Block Validation
Rewriting-history attacks can erase all valid records of blockchain-based systems, which is extremely devastating. To deter such attacks, we design a new smart contract-based secure model to make such attacks ineffective.
Shijie Zhang, Jong-Hyouk Lee
doaj +1 more source

