Results 241 to 250 of about 2,328,760 (311)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Versatile electroporation protocols enable reproducible CRISPR-RNP delivery across multiple primary mouse cells of the hematopoietic lineage. [PDF]

Front Immunol
Oh JH, Yang LF, Tanaka E, de Brito Monteiro L, Wi D, Archambault AS, Klein Geltink RI.   +6 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Advancing abdominal surgery recovery implementation: a unified framework for intensified recovery protocols by the EUropean PErioperative MEdical Networking collaborative. [PDF]

Front Surg
Ioannidis O, Bourtzinakou AA, Ramirez JM, Fabbri N, Ubieto JM, Feo CV, Pesce A, Rosetzka K, Arroyo A, Kocián P, Sánchez-Guillén L, Bellosta AP, Whitley A, Enguita AB, Teresa-Fernandéz M, Bitsianis S, Symeonidis S, Anestiadou E.   +17 more
europepmc   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Impact of dose allocation between high- and low-kV scans on virtual monoenergetic image quality in developmental dual-energy cone-beam CT for image-guided radiotherapy. [PDF]

J Appl Clin Med Phys
Keeler A, Layman L, Nguyen H, Luce J, Lehmann M, Roeske JC, Kang H.   +6 more
europepmc   +1 more source

Survey of practices around the measurement and replacement of calcium in paediatric major trauma. [PDF]

BMJ Paediatr Open
Hibberd O, Ranaweera M, Karageorgos S, Priddis K, Nijman RG, Tagg A, Hall D, Roland D.   +7 more
europepmc   +1 more source

National survey of protocols for day-case hip and knee arthroplasty in the UK. [PDF]

Bone Jt Open
Edwards TA, Large J, French JMR, Evans JT, Reed MR, Whitehouse MR, Day Case Collaborative, Ng ABY, Hosea A, Pradhan A, Date A, Charalambous A, Hunt A, Nakhla A, Firth AM, Metry A, Bolland BJ, Aliker C, Navaratnam DM, Waugh D, Ojelade E, Morriss F, Chadalavada G, Slade G, Higgins G, Neal-Smith G, Umer HM, Noone H, Rankin I, Muhammad I, Ahmed I, Morris J, Roberts J, Hill J, Quayle J, Topping J, Heylen J, Howard J, Moreau J, Roope J, Cumberworth J, Logishetty K, Hogan K, Khilla K, Syam K, Patel K, Kaushik Dash K, Sinan L, Jenner L, Girling L, Choi M, Andrews M, Gibby M, Petrie M, Wilson M, Mubashir Siddiqui M, Bhaskara NV, Flannery O, Kabariti R, Ashour R, Sinnerton R, Azeez S, Everett SJ, Miller S, Hermena S, Mehta S, Bannagham S, Mannan S, Khaleeq T, Maunganidze T, Petheram T, Ross Fraser T, Ng Z.   +72 more
europepmc   +1 more source

Handgrip and Acute Hemodynamic Responses During Cyclic Exercise: Randomized Clinical Trial. [PDF]

J Clin Hypertens (Greenwich)
Sacramento MSD, Souza PES, Leite JM, Barbosa RM, Santos ACND, Marques LDS, Oliveira AM, Petto J.   +7 more
europepmc   +1 more source

Analysis of digital vernier caliper <i>versus</i> digital thickness gauge consistency and user experience in measuring arterial wall thickness: implications for biomechanical assessment. [PDF]

Front Bioeng Biotechnol
Ciucanu CC, Russu E, Mureşan A, Asztalos AI, Ion AP, Bartus R, Arbănaşi EM, Mureşan AV, Radu CC, Hogea T, Chirilă TV, Voidǎzan ST, Arbănaşi EM.   +12 more
europepmc   +1 more source