Results 131 to 140 of about 5,165 (174)

Crystallization of exotoxin A from Pseudomonas aeruginosa

Journal of Molecular Biology, 1982
Abstract Exotoxin A from Pseudomonas aeruginosa has been crystallized in a form suitable for high resolution diffraction analysis. The crystals, grown in the presence of high concentrations of polyethylene glycol (20%, w/v) and of NaCl (1.5 m ), are monoclinic and contain one monomeric toxin molecule per asymmetric unit. The space group is P 2 1 ,
R J, Collier, D B, McKay
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Exotoxins of Pseudomonas aeruginosa. II. Concentration, Purification, and Characterization of Exotoxin A

Journal of Infectious Diseases, 1973
Exotoxin A of Pseudomonas aeruginosa (PA-103) could be concentrated readily by precipitation with zinc acetate and ammonium sulfate. The toxin was purified by column chromatography with use of DEAE-cellulose and Sephadex G-200. The final product contained about 8,000 mouse LD,,0/mg of protein.
P V, Liu, S, Yoshii, H, Hsieh
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Pseudomonas aeruginosa Exotoxin: Effect on Cell Cultures

Journal of Infectious Diseases, 1972
Abstract : An exotoxin, toxic to both mice and cultured cells, was isolated from cultures of Pseudomonas aeruginosa. Relatively small amounts of the exotoxin inhibited the uptake of uridine and amino acids by Vero cells. Within limits, this toxic action was reversible and could be inactivated by heating at 70 C or by proteolytic digestion, but it was ...
O R, Pavlovskis, F B, Gordon
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Pseudomonas aeruginosaExotoxin A

New England Journal of Medicine, 1980
Pseudomonas aeruginosa is an opportunistic pathogen that causes more than 100,000 infections in the United States each year. Pseudomonas infections are associated with considerable morbidity and mortality despite the use of modern antibiotics.1 Recognition of the limitations of existing therapy for pseudomonas disease has stimulated renewed interest in
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Pseudomonas Aeruginosa Exotoxin a Induces Apoptosis in Galleria Mellonella Hemocytes

SSRN Electronic Journal, 2022
The cellular immune response of the greater wax moth Galleria mellonella to Pseudomonas aeruginosa exotoxin A was investigated for the first time. The insects were challenged with a sublethal dose of exoA, and then hemocyte parameters were assessed. The analysis showed a statistically significant decrease in the total hemocyte count (THC), which was ...
Bartłomiej, Iwański, Magdalena, Mizerska-Kowalska, Mariola, Andrejko   +2 more
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Effects of proteases on the structure and activity of Pseudomonas aeruginosa exotoxin A

Infection and Immunity, 1981
The effects of various proteases on the enzymatic or biological activity and structure of exotoxin A from Pseudomonas aeruginosa were systematically studied. The toxin was extremely resistant to treatment with various enzymes. The lethality of the toxin disappeared upon treatment with P. aeruginosa protease and elastase, thermolysin, and trypsin with a
K, Morihara, Y, Sanai, H, Tsuzuki, H, Jyoyama, K, Hirose, J Y, Homma, I, Kato   +6 more
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Effect of Pseudomonas aeruginosa Exotoxin A on Inner Ear Function

Acta Oto-Laryngologica, 1997
Electrophysiological changes were studied in the albino rat following instillation of Pseudomonas aeruginosa exotoxin A into the middle ear cavity through the tympanic membrane. Hearing threshold was measured by a burst-elicited, frequency-specific auditory brainstem response (ABR) technique prior to exposure, then 24 and 48 h, 5 days, 2 and 4 weeks ...
M, Stenqvist, M, Anniko, A, Pettersson
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Pseudomonas aeruginosa exotoxin A

1997
Abstract Pseudomonas aeruginosa exotoxin A (PEA, MW 66 kDa, 613 amino acids, sequence accession number: PSEETA K01397) consists of three major domains (Allured et al. 1986), as indicated in Fig. 1. The N-terminal domain la (amino acids 1-252) binds to the ai-macroglobulin receptor at the cell surface (Kounnas et al.
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Toxoid from Exotoxin A of Pseudomonas aeruginosa: Preparation and Characterization

Journal of Infectious Diseases, 1982
Toxoid was prepared by treating exotoxin A of Pseudomonas aeruginosa with formalin. Coincubation of exotoxin A for two to four weeks at 37 degrees C with 0.5% formalin and 10(-3) M lysine followed by one to two weeks of storage in the absence of these reagents reduced cytotoxicity, preserved antigenicity, and minimized subsequent reversion of the ...
M, Pollack, R K, Prescott
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Regulation of Pseudomonas Aeruginosa Exotoxin a Synthesis

2004
Exotoxin A (ETA) is one of the most toxic virulence factors produced by Pseudomonas aeruginosa 72: Both clinical and experimental animal studies indicate clearly the importance of ETA in the pathogenesis of different P. aeruginosa infections. Most P. aeruginosa clinical isolates produce ETA9, 23, 47.
Abdul N. Hamood, Jane A. Colmer-Hamood, Nancy L. Carty   +2 more
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